ZFIN ID: ZDB-PUB-200624-3
A bright and high-performance genetically encoded Ca2+ indicator based on mNeonGreen fluorescent protein
Zarowny, L., Aggarwal, A., Rutten, V.M.S., Kolb, I., Project, T.G., Patel, R., Huang, H.Y., Chang, Y.F., Phan, T., Kanyo, R., Ahrens, M.B., Allison, W.T., Podgorski, K., Campbell, R.E.
Date: 2020
Source: ACS sensors   5(7): 1959-1968 (Journal)
Registered Authors: Ahrens, Misha, Allison, Ted
Keywords: none
MeSH Terms: none
PubMed: 32571014 Full text @ ACS Sens
ABSTRACT
Genetically encodable calcium ion (Ca2+) indicators (GECIs) based on green fluorescent proteins (GFP) are powerful tools for imaging of cell signaling and neural activity in model organisms. Following almost two decades of steady improvements in the Aequorea victoria GFP (avGFP)-based GCaMP series of GECIs, the performance of the most recent generation (i.e., GCaMP7) may have reached its practical limit due to the inherent properties of GFP. In an effort to sustain the steady progression towards ever-improved GECIs, we undertook the development of a new GECI based on the bright monomeric GFP, mNeonGreen (mNG). The resulting indicator, mNG-GECO1, is 60% brighter than GCaMP6s in vitro and provides comparable performance as demonstrated by imaging Ca2+ dynamics in cultured cells, primary neurons, and in vivo in larval zebrafish. These results suggest that mNG-GECO1 is a promising next-generation GECI that could inherit the mantle of GCaMP and allow the steady improvement of GECIs to continue for generations to come.
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