PUBLICATION
NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
- Authors
- Mattern, K., von Trotha, J.W., Erfle, P., Köster, R.W., Dietzel, A.
- ID
- ZDB-PUB-200618-6
- Date
- 2020
- Source
- Communications biology 3: 311 (Journal)
- Registered Authors
- Köster, Reinhard W., von Trotha, Jakob
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Brain/diagnostic imaging*
- Calcium Signaling
- Computer-Aided Design
- Equipment Design
- Glass
- Hydrodynamics
- Lab-On-A-Chip Devices*
- Larva
- Microfluidics/instrumentation
- Microscopy/instrumentation*
- Microscopy/methods
- Zebrafish*/genetics
- PubMed
- 32546816 Full text @ Commun Biol
Citation
Mattern, K., von Trotha, J.W., Erfle, P., Köster, R.W., Dietzel, A. (2020) NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish. Communications biology. 3:311.
Abstract
While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping