PUBLICATION
lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway
- Authors
- Zhang, Q., Li, T., Wang, Z., Kuang, X., Shao, N., Lin, Y.
- ID
- ZDB-PUB-200618-12
- Date
- 2020
- Source
- Journal of Cellular and Molecular Medicine 24(14): 8236-8247 (Journal)
- Registered Authors
- Keywords
- IGF-1, IGF-1R, angiogenesis, breast cancer, lncRNA NR2F1-AS1
- MeSH Terms
-
- Animals
- Breast Neoplasms/genetics*
- Breast Neoplasms/metabolism*
- Breast Neoplasms/pathology
- COUP Transcription Factor I/genetics*
- Cell Line, Tumor
- Disease Models, Animal
- Extracellular Signal-Regulated MAP Kinases/metabolism*
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Insulin-Like Growth Factor I/metabolism*
- Neovascularization, Pathologic/genetics
- Neovascularization, Pathologic/metabolism
- RNA, Long Noncoding/genetics*
- Receptor, IGF Type 1/metabolism*
- Signal Transduction
- Zebrafish
- PubMed
- 32548873 Full text @ J. Cell. Mol. Med.
Citation
Zhang, Q., Li, T., Wang, Z., Kuang, X., Shao, N., Lin, Y. (2020) lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway. Journal of Cellular and Molecular Medicine. 24(14):8236-8247.
Abstract
Long non-coding RNAs (lncRNAs) take various effects in cancer mostly through sponging with microRNAs (miRNAs). lncRNA NR2F1-AS1 is found to promote tumour progression in hepatocellular carcinoma, endometrial cancer and thyroid cancer. However, the role of lncRNA NR2F1-AS1 in breast cancer angiogenesis remains unknown. In this study, we found lncRNA NR2F1-AS1 was positively related with CD31 and CD34 in breast cancer through Pearson's correlation analysis, while lncRNA NR2F1-AS1 transfection promoted human umbilical vascular endothelial cell (HUVEC) tube formation. In breast cancer cells, lncRNA NR2F1-AS1 enhanced the HUVEC proliferation, tube formation and migration ability through tumour-conditioned medium (TCM). In zebrafish model, lncRNA NR2F1-AS1 increased the breast cancer cell-related neo-vasculature and subsequently promoted the breast cancer cell metastasis. In mouse model, lncRNA NR2F1-AS1 promoted the tumour vessel formation, increased the micro vessel density (MVD) and then induced the growth of primary tumour. Mechanically, lncRNA NR2F1-AS1 increased insulin-like growth factor-1 (IGF-1) expression through sponging miRNA-338-3p in breast cancer cells and then activated the receptor of IGF-1 (IGF-1R) and extracellular signal-regulated kinase (ERK) pathway in HUVECs. These results indicated that lncRNA NR2F1-AS1 could promote breast cancer angiogenesis through IGF-1/IGF-1R/ERK pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping