PUBLICATION
A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia
- Authors
- Kuncha, S.K., Mazeed, M., Singh, R., Kattula, B., Routh, S.B., Sankaranarayanan, R.
- ID
- ZDB-PUB-200522-31
- Date
- 2018
- Source
- Nature communications 9: 511 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Sequence Homology, Amino Acid
- Protein Conformation, alpha-Helical
- Transfer RNA Aminoacylation/genetics*
- Threonine/chemistry*
- Threonine/genetics
- Threonine/metabolism
- Animals
- Crystallography, X-Ray
- Humans
- Aminoacyltransferases/chemistry*
- Aminoacyltransferases/genetics
- Aminoacyltransferases/metabolism
- Biological Evolution
- Bacteria/genetics
- Bacteria/metabolism
- RNA, Transfer, Amino Acyl/chemistry*
- RNA, Transfer, Amino Acyl/genetics
- RNA, Transfer, Amino Acyl/metabolism
- Protein Conformation, beta-Strand
- Protein Binding
- Protein Interaction Domains and Motifs
- Gene Expression
- Recombinant Proteins/chemistry
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Cloning, Molecular
- Apicomplexa/genetics
- Apicomplexa/metabolism
- Amino Acid Sequence
- Alanine/chemistry*
- Alanine/genetics
- Alanine/metabolism
- Binding Sites
- Substrate Specificity
- Models, Molecular
- Sequence Alignment
- PubMed
- 29410408 Full text @ Nat. Commun.
Citation
Kuncha, S.K., Mazeed, M., Singh, R., Kattula, B., Routh, S.B., Sankaranarayanan, R. (2018) A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia. Nature communications. 9:511.
Abstract
D-aminoacyl-tRNA deacylase (DTD), a bacterial/eukaryotic trans-editing factor, removes D-amino acids mischarged on tRNAs and achiral glycine mischarged on tRNAAla. An invariant cross-subunit Gly-cisPro motif forms the mechanistic basis of L-amino acid rejection from the catalytic site. Here, we present the identification of a DTD variant, named ATD (Animalia-specific tRNA deacylase), that harbors a Gly-transPro motif. The cis-to-trans switch causes a "gain of function" through L-chiral selectivity in ATD resulting in the clearing of L-alanine mischarged on tRNAThr(G4•U69) by eukaryotic AlaRS. The proofreading activity of ATD is conserved across diverse classes of phylum Chordata. Animalia genomes enriched in tRNAThr(G4•U69) genes are in strict association with the presence of ATD, underlining the mandatory requirement of a dedicated factor to proofread tRNA misaminoacylation. The study highlights the emergence of ATD during genome expansion as a key event associated with the evolution of Animalia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping