PUBLICATION
Heterogeneous T cell motility behaviors emerge from a coupling between speed and turning in vivo
- Authors
- Jerison, E.R., Quake, S.R.
- ID
- ZDB-PUB-200520-14
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Keywords
- T cells, cell motility, light sheet microscopy, persistent random walk, physics of living systems, zebrafish
- Datasets
- GEO:GSE137770
- MeSH Terms
-
- Animals
- Cell Movement*
- Light
- Microscopy
- Models, Biological*
- T-Lymphocytes/physiology
- Zebrafish/physiology*
- PubMed
- 32427565 Full text @ Elife
Citation
Jerison, E.R., Quake, S.R. (2020) Heterogeneous T cell motility behaviors emerge from a coupling between speed and turning in vivo. eLIFE. 9:.
Abstract
T cells in vivo migrate primarily via undirected random walks, but it remains unresolved how these random walks generate an efficient search. Here, we use light sheet microscopy of T cells in the larval zebrafish as a model system to study motility across large populations of cells over hours in their native context. We show that cells do not perform Levy flight; rather, there is substantial cell-to-cell variability in speed, which persists over timespans of a few hours. This variability is amplified by a correlation between speed and directional persistence, generating a characteristic cell behavioral manifold that is preserved under a perturbation to cell speeds, and seen in Mouse T cells and Dictyostelium. Together, these effects generate a broad range of length scales over which cells explore in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping