PUBLICATION
A feasibility study for noninvasive measurement of shear wave speed in live zebrafish
- Authors
- Zhang, X., Zhang, A.X., Zhou, B., Xu, X.
- ID
- ZDB-PUB-200518-16
- Date
- 2020
- Source
- Ultrasonics 107: 106170 (Journal)
- Registered Authors
- Xu, Xiaolei
- Keywords
- In vivo zebrafish testing, Shear wave speed, Ultrasound vibro-elastography, Zebrafish
- MeSH Terms
-
- Animals
- Elasticity Imaging Techniques/methods*
- Feasibility Studies
- Models, Animal
- Tail/diagnostic imaging*
- Vibration
- Zebrafish*
- PubMed
- 32417695 Full text @ Ultrasonics
Citation
Zhang, X., Zhang, A.X., Zhou, B., Xu, X. (2020) A feasibility study for noninvasive measurement of shear wave speed in live zebrafish. Ultrasonics. 107:106170.
Abstract
Zebrafish are being increasingly used as animal models for human diseases such as cardiomyopathy and neuroblastoma. Owing to a nearly fully sequenced genome and efficient genetics/chemical genetics, zebrafish open new research opportunities for human diseases research. The purpose of this study was to develop zebrafish ultrasound vibro-elastography (ZUVE) for measuring the shear wave speed of zebrafish. An adult female zebrafish was anesthetized for three minutes for the ZUVE testing. A 0.1 s gentle harmonic vibration was generated on the tail using a sphere tip indenter with 3 mm diameter. Shear wave propagation in the zebrafish was measured using a high frequency 18 MHz ultrasound probe. Shear wave speeds were measured at 300, 400, and 500 Hz. Shear wave speeds were, respectively, 3.13 ± 1.20 (m/s) for 300 Hz, 4.28 ± 1.36 (m/s) for 400 Hz, and 5.07 ± 1.45 (m/s) for 500 Hz for zebrafish 1 in a region of interest (ROI) which covered the central body. The shear wave speed dispersions were similar for four zebrafish and shear wave speeds ranged between 2.5 (m/s) and 5 (m/s) from 300 Hz to 500 Hz. The experimental setup and testing for a zebrafish lasted less than three minutes. All tested zebrafish were alive after testing. ZUVE is safe, fast, and noninvasive, making the testing of elastic properties of zebrafish feasible.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping