PUBLICATION
Surface Modification of Magnetic Nanoparticles by Carbon-Coating Can Increase Its Biosafety: Evidences from Biochemical and Neurobehavioral Tests in Zebrafish
- Authors
- Malhotra, N., Audira, G., Chen, J.R., Siregar, P., Hsu, H.S., Lee, J.S., Ger, T.R., Hsiao, C.D.
- ID
- ZDB-PUB-200515-1
- Date
- 2020
- Source
- Molecules 25(9): (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- behavior analysis, carbon magnetic nanoparticles, ecotoxicity, neurotransmitters, phenomic analysis, zebrafish
- MeSH Terms
-
- Animals
- Behavior Rating Scale
- Brain/drug effects*
- Brain/metabolism
- Carbon/chemistry*
- Catecholamines/metabolism
- Cluster Analysis
- Female
- Hydrocortisone/metabolism
- Magnetic Resonance Imaging
- Magnetics
- Magnetite Nanoparticles/chemistry*
- Magnetite Nanoparticles/toxicity*
- Magnetite Nanoparticles/ultrastructure
- Male
- Metallothionein/metabolism
- Microscopy, Electron, Transmission
- Neurotransmitter Agents/metabolism
- Oxidative Stress/drug effects*
- Principal Component Analysis
- Reactive Oxygen Species/metabolism
- Spectroscopy, Fourier Transform Infrared
- Spectrum Analysis, Raman
- X-Ray Diffraction
- Zebrafish/metabolism*
- Zebrafish/physiology
- PubMed
- 32403340 Full text @ Molecules
Citation
Malhotra, N., Audira, G., Chen, J.R., Siregar, P., Hsu, H.S., Lee, J.S., Ger, T.R., Hsiao, C.D. (2020) Surface Modification of Magnetic Nanoparticles by Carbon-Coating Can Increase Its Biosafety: Evidences from Biochemical and Neurobehavioral Tests in Zebrafish. Molecules. 25(9):.
Abstract
Recently, magnetic nanoparticles (MNPs) have gained much attention in the field of biomedical engineering for therapeutic as well as diagnostic purposes. Carbon magnetic nanoparticles (C-MNPs) are a class of MNPs categorized as organic nanoparticles. C-MNPs have been under considerable interest in studying in various applications such as magnetic resonance imaging, photothermal therapy, and intracellular transportof drugs. Research work is still largely in progress for testing the efficacy of C-MNPs on the theranostics platform in cellular studies and animal models. In this study, we evaluated the neurobehavioral toxicity parameters on the adult zebrafish (Danio rerio) at either low (1 ppm) or high (10 ppm) concentration level of C-MNPs over a period of two weeks by waterborne exposure. The physical properties of the synthesized C-MNPs were characterized by transmission electron microscopy, Raman, and XRD spectrum characterization. Multiple behavior tests for the novel tank, mirror biting, predator avoidance, conspecific social interaction, shoaling, and analysis of biochemical markers were also conducted to elucidate the corresponding mechanism. Our data demonstrate the waterborne exposure of C-MNPs is less toxic than the uncoated MNPs since neither low nor high concentration C-MNPs elicit toxicity response in behavioral and biochemical tests in adult zebrafish. The approach combining biochemical and neurobehavioral approaches would be helpful for understanding C-MNPs association affecting the bioavailability, biosafety, interaction, and uptake of these C-MNPs in the living organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping