PUBLICATION
Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish
- Authors
- Singh, S.P., Ninov, N.
- ID
- ZDB-PUB-200403-95
- Date
- 2020
- Source
- Methods in molecular biology (Clifton, N.J.) 2128: 159-179 (Review)
- Registered Authors
- Ninov, Nikolay, Singh, Sumeet Pal
- Keywords
- Brainbow, Heterogeneity, Lineage tracing, Quiescence, Single cell
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Lineage
- Cell Proliferation*
- Cell Tracking/methods*
- Cloning, Molecular/methods
- Color
- Genes, Reporter
- Image Processing, Computer-Assisted/methods*
- Insulin-Secreting Cells/chemistry
- Insulin-Secreting Cells/cytology*
- Integrases
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Microscopy, Confocal/methods*
- Models, Animal
- Staining and Labeling/methods*
- Zebrafish
- PubMed
- 32180193 Full text @ Meth. Mol. Biol.
Citation
Singh, S.P., Ninov, N. (2020) Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2128:159-179.
Abstract
During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping