ZFIN ID: ZDB-PUB-200403-54
Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood
Schwarzer, S., Asokan, N., Bludau, O., Kuscha, V., Kaslin, J., Hans, S.
Date: 2020
Source: Development (Cambridge, England)   147(7): (Journal)
Registered Authors: Hans, Stefan, Kaslin, Jan, Kuscha, Veronika
Keywords: Inner ear, Neuronal stem cells, PNS, Zebrafish
MeSH Terms:
  • Adult Stem Cells/cytology
  • Adult Stem Cells/physiology*
  • Aging/physiology
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cell Differentiation/genetics
  • Ear, Inner/cytology
  • Ear, Inner/physiology*
  • Embryo, Nonmammalian
  • Ganglia, Sensory/cytology*
  • Ganglia, Sensory/physiology
  • Gene Expression Regulation, Developmental
  • Hair Cells, Auditory/metabolism
  • Hair Cells, Auditory/physiology*
  • Larva
  • Nerve Tissue Proteins/metabolism
  • Nestin/metabolism
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Neural Stem Cells/physiology*
  • Neurogenesis/physiology*
  • Sensory Receptor Cells/cytology
  • Sensory Receptor Cells/physiology
  • Stem Cell Niche/physiology
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
  • Zebrafish*/metabolism
PubMed: 32165493 Full text @ Development
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ABSTRACT
The vertebrate inner ear employs sensory hair cells and neurons to mediate hearing and balance. In mammals, damaged hair cells and neurons are not regenerated. In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. However, it is unknown whether new sensory neurons are also formed in the adult zebrafish statoacoustic ganglion (SAG), the sensory ganglion connecting the inner ear to the brain. Using transgenic lines and marker analysis, we identify distinct cell populations and anatomical landmarks in the juvenile and adult SAG. In particular, we analyze a Neurod/Nestin-positive progenitor pool that produces large amounts of new neurons at juvenile stages, which transitions to a quiescent state in the adult SAG. Moreover, BrdU pulse chase experiments reveal the existence of a proliferative but otherwise marker-negative cell population that replenishes the Neurod/Nestin-positive progenitor pool at adult stages. Taken together, our study represents the first comprehensive characterization of the adult zebrafish SAG showing that zebrafish, in sharp contrast to mammals, display continued neurogenesis in the SAG well beyond embryonic and larval stages.
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