PUBLICATION

A calibrated optogenetic toolbox of stable zebrafish opsin lines

Authors
Antinucci, P., Dumitrescu, A., Deleuze, C., Morley, H.J., Leung, K., Hagley, T., Kubo, F., Baier, H., Bianco, I.H., Wyart, C.
ID
ZDB-PUB-200403-195
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Antinucci, Paride, Baier, Herwig, Bianco, Isaac, Kubo, Fumi, Wyart, Claire
Keywords
neuroscience, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Calibration
  • Chlorides/metabolism
  • Escape Reaction
  • Motor Neurons/physiology
  • Opsins/genetics*
  • Optogenetics*
  • Proton Pumps/physiology
  • Rhodopsin/physiology
  • Trigeminal Ganglion/embryology
  • Zebrafish/embryology
PubMed
32216873 Full text @ Elife
Abstract
Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes