ZFIN ID: ZDB-PUB-200403-146
Rac2 Regulates the Migration of T Lymphoid Progenitors to the Thymus during Zebrafish Embryogenesis
Lu, X., Zhang, Y., Liu, F., Wang, L.
Date: 2020
Source: Journal of immunology (Baltimore, Md. : 1950)   204(9): 2447-2454 (Journal)
Registered Authors: Liu, Feng, Wang, Lu
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/immunology
  • Animals, Genetically Modified/metabolism
  • Bone Marrow/immunology
  • Bone Marrow/metabolism
  • Cell Differentiation/immunology
  • Cell Movement/immunology*
  • Embryonic Development/immunology*
  • Lymphoid Progenitor Cells/immunology
  • Lymphoid Progenitor Cells/metabolism*
  • Lymphopoiesis/immunology
  • Proto-Oncogene Proteins c-akt/immunology
  • Proto-Oncogene Proteins c-akt/metabolism
  • Signal Transduction/immunology
  • Thymus Gland/immunology
  • Thymus Gland/metabolism*
  • Zebrafish/immunology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism*
  • p21-Activated Kinases/immunology
  • p21-Activated Kinases/metabolism
  • rac GTP-Binding Proteins/immunology
  • rac GTP-Binding Proteins/metabolism*
  • rho GTP-Binding Proteins/immunology
  • rho GTP-Binding Proteins/metabolism
PubMed: 32198141 Full text @ J. Immunol.
ABSTRACT
The caudal hematopoietic tissue in zebrafish, the equivalent to the fetal liver in mammals, is an intermediate hematopoietic niche for the maintenance and differentiation of hematopoietic stem and progenitor cells before homing to the thymus and kidney marrow. As one of the ultimate hematopoietic organs, the thymus sustains T lymphopoiesis, which is essential for adaptive immune system. However, the mechanism of prethymic T lymphoid progenitors migrating to the thymus remains elusive. In this study, we identify an Rho GTPase Rac2 as a modulator of T lymphoid progenitor homing to the thymus in zebrafish. rac2-Deficient embryos show the inability of T lymphoid progenitors homing to the thymus because of defective cell-autonomous motility. Mechanistically, we demonstrate that Rac2 regulates homing of T lymphoid progenitor through Pak1-mediated AKT pathway. Taken together, our work reveals an important function of Rac2 in directing T lymphoid progenitor migration to the thymus during zebrafish embryogenesis.
ADDITIONAL INFORMATION