PUBLICATION
A virtual reality system to analyze neural activity and behavior in adult zebrafish
- Authors
- Huang, K.H., Rupprecht, P., Frank, T., Kawakami, K., Bouwmeester, T., Friedrich, R.W.
- ID
- ZDB-PUB-200304-32
- Date
- 2020
- Source
- Nature Methods 17(3): 343-351 (Journal)
- Registered Authors
- Friedrich, Rainer, Kawakami, Koichi
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal*
- Brain/diagnostic imaging*
- Brain Mapping
- Cognition
- Decision Making
- Female
- Male
- Motor Skills
- Neurons/physiology*
- Probability
- Social Behavior
- Swimming
- User-Computer Interface*
- Virtual Reality*
- Zebrafish
- PubMed
- 32123394 Full text @ Nat. Methods
Citation
Huang, K.H., Rupprecht, P., Frank, T., Kawakami, K., Bouwmeester, T., Friedrich, R.W. (2020) A virtual reality system to analyze neural activity and behavior in adult zebrafish. Nature Methods. 17(3):343-351.
Abstract
Virtual realities are powerful tools to analyze and manipulate interactions between animals and their environment and to enable measurements of neuronal activity during behavior. In many species, however, optical access to the brain and/or the behavioral repertoire are limited. We developed a high-resolution virtual reality for head-restrained adult zebrafish, which exhibit cognitive behaviors not shown by larvae. We noninvasively measured activity throughout the dorsal telencephalon by multiphoton calcium imaging. Fish in the virtual reality showed regular swimming patterns and were attracted to animations of conspecifics. Manipulations of visuo-motor feedback revealed neurons that responded selectively to the mismatch between the expected and the actual visual consequences of motor output. Such error signals were prominent in multiple telencephalic areas, consistent with models of predictive processing. A virtual reality system for adult zebrafish therefore provides opportunities to analyze neuronal processing mechanisms underlying higher brain functions including decision making, associative learning, and social interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping