PUBLICATION
Low doses of ionizing radiation enhance angiogenesis and consequently accelerate post-embryonic development but not regeneration in zebrafish
- Authors
- Marques, F.G., Carvalho, L., Sousa, J.S., Rino, J., Diegues, I., Poli, E., Pina, F., Saúde, L., Constantino Rosa Santos, S.
- ID
- ZDB-PUB-200225-13
- Date
- 2020
- Source
- Scientific Reports 10: 3137 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animal Fins/physiology*
- Animal Fins/radiation effects*
- Animals
- Animals, Genetically Modified
- Cell Separation
- Endothelial Cells/physiology*
- Endothelial Cells/radiation effects
- Enzyme Inhibitors
- Flow Cytometry
- Larva/physiology
- Larva/radiation effects
- Morphogenesis
- Neovascularization, Physiologic/radiation effects*
- Radiation, Ionizing*
- Regeneration/radiation effects*
- Signal Transduction
- Transcription Factors
- Vascular Endothelial Growth Factor Receptor-2/antagonists & inhibitors
- Zebrafish/growth & development*
- Zebrafish Proteins/antagonists & inhibitors
- PubMed
- 32081879 Full text @ Sci. Rep.
Citation
Marques, F.G., Carvalho, L., Sousa, J.S., Rino, J., Diegues, I., Poli, E., Pina, F., Saúde, L., Constantino Rosa Santos, S. (2020) Low doses of ionizing radiation enhance angiogenesis and consequently accelerate post-embryonic development but not regeneration in zebrafish. Scientific Reports. 10:3137.
Abstract
Low doses of ionizing radiation (LDIR) activate endothelial cells inducing angiogenesis. In zebrafish, LDIR induce vessel formation in the sub-intestinal vessels during post-embryonic development and enhance the inter-ray vessel density in adult fin regeneration. Since angiogenesis is a crucial process involved in both post-embryonic development and regeneration, herein we aimed to understand whether LDIR accelerate these physiological conditions. Our data show that LDIR upregulate the gene expression of several pro-angiogenic molecules, such as flt1, kdr, angpt2a, tgfb2, fgf2 and cyr61in sorted endothelial cells from zebrafish larvae and this effect was abrogated by using a vascular endothelial growth factor receptor (VEGFR)-2 tyrosine kinase inhibitor. Irradiated zebrafish present normal indicators of developmental progress but, importantly LDIR accelerate post-embryonic development in a VEGFR-2 dependent signaling. Furthermore, our data show that LDIR do not accelerate regeneration after caudal fin amputation and the gene expression of the early stages markers of regeneration are not modulated by LDIR. Even though regeneration is considered as a recapitulation of embryonic development and LDIR induce angiogenesis in both conditions, our findings show that LDIR accelerate post-embryonic development but not regeneration. This highlights the importance of the physiological context for a specific phenotype promoted by LDIR.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping