PUBLICATION

The BMP ligand Pinhead together with Admp supports the robustness of embryonic patterning

Authors
Yan, Y., Ning, G., Li, L., Liu, J., Yang, S., Cao, Y., Wang, Q.
ID
ZDB-PUB-200221-16
Date
2019
Source
Science advances   5: eaau6455 (Journal)
Registered Authors
Wang, Qiang
Keywords
none
MeSH Terms
  • Signal Transduction
  • Zebrafish Proteins/metabolism*
  • Embryo, Nonmammalian
  • Body Patterning*
  • Zebrafish/metabolism
  • Carrier Proteins/metabolism*
  • Smad Proteins/metabolism
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Animals
  • Bone Morphogenetic Proteins/metabolism*
  • Ligands
(all 12)
PubMed
32064309 Full text @ Sci Adv
Abstract
Vertebrate embryonic dorsoventral axis is robustly stable in the face of variations in bone morphogenetic protein (BMP) signaling. However, the molecular mechanism behind this robustness remains uncharacterized. In this study, we show that zebrafish Pinhead, together with Admp, plays an important compensatory role in ensuring the robustness of axial patterning through fine-tuning of BMP signaling. pinhead encodes a BMP-like ligand expressed in the ventrolateral margin of the early gastrula. Transcription of pinhead and admp is under opposing regulation, where pinhead depletion results in a compensatory increase in admp transcription and vice versa, leading to normal axis formation in pinhead or admp mutants. Expression of pinhead and admp is directly repressed by the BMP/Smad pathway. When BMP signals were inhibited or excessively activated, pinhead/admp expression changed accordingly, allowing for self-regulation. Thus, this study reveals a negative feedback loop between BMP signaling and pinhead/admp that effectively stabilizes embryonic patterning by buffering against fluctuations in BMP signaling.
Genes / Markers
Figures
No images available
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
TUchemical treatment: dorsomorphinShield
TUchemical treatment: dorsomorphinShield
TUchemical treatment: DMH1Shield
TUchemical treatment: DMH1Shield
TU + MO1-bmp2bstandard conditionsShield
TU + MO1-bmp2bstandard conditionsShield
TU + MO1-pnhdstandard conditions30%-epiboly
TU + MO2-admpstandard conditions30%-epiboly
admpioz10/ioz10 (TU)standard conditions30%-epiboly
admpioz10/ioz10 (TU)standard conditionsSphere to Shield
1 - 10 of 27
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ioz10
    		Small Deletion
    ioz11
      		Small Deletion
      pt510TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Fish
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
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        Citation
        Yan, Y., Ning, G., Li, L., Liu, J., Yang, S., Cao, Y., Wang, Q. (2019) The BMP ligand Pinhead together with Admp supports the robustness of embryonic patterning. Science advances. 5:eaau6455.