PUBLICATION

Evaluation of Antioxidant Activity of Spice-Derived Phytochemicals Using Zebrafish

Authors
Endo, Y., Muraki, K., Fuse, Y., Kobayashi, M.
ID
ZDB-PUB-200213-2
Date
2020
Source
International Journal of Molecular Sciences   21(3): (Journal)
Registered Authors
Endo, Yuka, Fuse, Yuji, Kobayashi, Makoto
Keywords
6-MSITC, H2O2, NaAsO2, Nrf2, antioxidant activity, dietary phytochemical, food ingredient, oxidative stress, spice, zebrafish
MeSH Terms
  • Curcumin/pharmacology*
  • Arsenites/toxicity
  • Antioxidants/pharmacology*
  • Spices*
  • Larva/drug effects
  • Allyl Compounds/pharmacology*
  • Drug Evaluation, Preclinical/methods*
  • Hydrogen Peroxide/toxicity
  • Sulfides/pharmacology*
  • High-Throughput Screening Assays/methods*
  • Zebrafish Proteins/metabolism
  • Zebrafish
  • NF-E2-Related Factor 2/metabolism
  • Quercetin/pharmacology*
  • Animals
(all 15)
PubMed
32046157 Full text @ Int. J. Mol. Sci.
Abstract
Various dietary phytochemicals seem to display antioxidant activity through the NF-E2-related factor 2 (Nrf2) pathway. However, few studies have demonstrated its antioxidant effect and Nrf2 dependency at the animal level. We constructed a zebrafish-based assay system to analyze the in vivo antioxidant activity of phytochemicals and examined the activity of 10 phytochemicals derived from spices, using this system as a pilot study. Hydrogen peroxide and arsenite were used as oxidative stressors, and Nrf2 dependency was genetically analyzed using an Nrf2-mutant zebrafish line. The activities of curcumin, diallyl trisulfide and quercetin were involved in the reduction of hydrogen peroxide toxicity, while those of cinnamaldehyde, isoeugenol and 6-(methylsulfinyl)hexyl isothiocyanate were involved in the reduction of arsenite toxicity. The antioxidant activities of these phytochemicals were all Nrf2 dependent, with the exception of cinnamaldehyde, which showed strong antioxidant effects even in Nrf2-mutant zebrafish. In summary, we succeeded in constructing an assay system to evaluate the in vivo antioxidant activity of various phytochemicals using zebrafish larvae. Using this system, we found that each spice-derived phytochemical has its own specific property and mechanism of antioxidant action.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Endo, Y., Muraki, K., Fuse, Y., Kobayashi, M. (2020) Evaluation of Antioxidant Activity of Spice-Derived Phytochemicals Using Zebrafish. International Journal of Molecular Sciences. 21(3):.