PUBLICATION

PDGF signaling from pharyngeal pouches promotes arch artery morphogenesis

Authors
Mao, A., Zhang, M., Liu, J., Cao, Y., Wang, Q.
ID
ZDB-PUB-200125-2
Date
2019
Source
Journal of genetics and genomics = Yi chuan xue bao   46(12): 551-559 (Journal)
Registered Authors
Wang, Qiang
Keywords
PDGFαa, PDGFαb, Pharyngeal arch artery, Pharyngeal pouch, Tissue-tissue interaction, Zebrafish
MeSH Terms
  • Animals
  • Branchial Region/embryology*
  • Branchial Region/metabolism*
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental/physiology
  • Nitroreductases/genetics
  • Nitroreductases/metabolism
  • Phosphorylation
  • Platelet-Derived Growth Factor/metabolism*
  • Signal Transduction/physiology
  • Zebrafish
PubMed
31974005 Full text @ J. Genet. Genomics
Abstract
The great vessels of the heart originate from the pharyngeal arch arteries (PAAs). Anomalies of the PAAs often occur together with pharyngeal pouch malformations, but the reasons for this phenomenon are not fully understood. In the current study, we show that platelet-derived growth factor (PDGF) signaling derived from the pharyngeal pouches plays an important function in PAA vasculogenesis. During PAA development in zebrafish embryos, pdgfαa and pdgfαb are expressed in the developing pharyngeal pouches. Results from loss-of-function experiments revealed a critical role of these genes in PAA formation. We found that nitroreductase (NTR)-mediated pouch ablation distinctly decreased PDGF receptor tyrosine phosphorylation, yielding a severe loss of PAAs. Importantly, pouch-specific overexpression of pdgfαa in pdgfαa-/-; pdgfαb-/- mutants significantly relieved the PAA defects, which indicated a primary role of pharyngeal pouch-expressed PDGF ligands in signal activation and PAA morphogenesis. Our findings further showed that PDGF signaling was indispensable for the proliferation of PAA angioblasts. Together, these results established a role for PDGFαa- and PDGFαb-mediated tissue-tissue interaction during PAA development.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping