PUBLICATION
Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
- Authors
- Rey-Campos, M., Moreira, R., Romero, A., Medina-Gali, R.M., Novoa, B., Gasset, M., Figueras, A.
- ID
- ZDB-PUB-200118-14
- Date
- 2020
- Source
- Biomolecules 10(1): (Journal)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- Illumina, Mytilus galloprovincialis, chemotaxis, hemocytes, myticin C, regeneration, tissue injury, wound healing
- MeSH Terms
-
- Animals
- Antimicrobial Cationic Peptides/genetics
- Antimicrobial Cationic Peptides/metabolism*
- Antimicrobial Cationic Peptides/pharmacology*
- Bivalvia/genetics
- Bivalvia/physiology*
- Blood Proteins/genetics
- Blood Proteins/metabolism*
- Blood Proteins/pharmacology*
- Cell Line
- Hemocytes/metabolism
- Humans
- Keratinocytes/drug effects
- Regeneration/drug effects
- Transcriptome*/drug effects
- Wound Healing*/drug effects
- Zebrafish
- PubMed
- 31947557 Full text @ Biomolecules
Citation
Rey-Campos, M., Moreira, R., Romero, A., Medina-Gali, R.M., Novoa, B., Gasset, M., Figueras, A. (2020) Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C. Biomolecules. 10(1):.
Abstract
Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilusgalloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tissue injury. Therefore, the main objective of this work was to characterize the hemocytes' transcriptomic response after a myticin C treatment, in order to understand the molecular changes induced by this cytokine-like molecule. The transcriptome analysis revealed the modulation of genes related to cellular movement, such as myosin, transgelin, and calponin-like proteins, in agreement with results of functional assays, where an implication of myticin C in the in vitro activation of hemocytes and migration was evidenced. This was also observed in vivo after a tissue injury, when hemocytes, with high concentrations of myticin C, migrated to the damaged area to heal the wound. All these properties allowed us to think about the biotechnological application of these molecules as wound healers. Human keratinocytes and larvae zebrafish models were used to confirm this hypothesis. Accelerated regeneration after a wound or tail fin amputation was observed after treatment with the myticin C peptide, supporting the chemotactic and healing activity of myticin C.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping