PUBLICATION
microRNA-1 Regulates NCC Migration and Differentiation by Targeting sec63
- Authors
- Wang, D., Weng, Y., Guo, S., Qin, W., Ni, J., Yu, L., Zhang, Y., Zhao, Q., Ben, J., Ma, J.
- ID
- ZDB-PUB-191123-7
- Date
- 2019
- Source
- International journal of biological sciences 15: 2538-2547 (Journal)
- Registered Authors
- Keywords
- Cranial Defect, Neural Crest Cells, Sec63, iTRAQ, microRNA
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Movement
- Computational Biology
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- In Situ Hybridization
- Maxillofacial Development/genetics*
- MicroRNAs/genetics
- MicroRNAs/metabolism
- MicroRNAs/physiology*
- Neural Crest/cytology
- Neural Crest/growth & development*
- Neural Crest/metabolism
- Proteomics
- Skull/embryology
- Time-Lapse Imaging
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 31754327 Full text @ Int. J. Biol. Sci.
Citation
Wang, D., Weng, Y., Guo, S., Qin, W., Ni, J., Yu, L., Zhang, Y., Zhao, Q., Ben, J., Ma, J. (2019) microRNA-1 Regulates NCC Migration and Differentiation by Targeting sec63. International journal of biological sciences. 15:2538-2547.
Abstract
Background/Aims: Neural crest cells play a vital role in craniofacial development, microRNA-1 (miR-1) is essential in development and disease of the cardiac and skeletal muscle, the objective of our study is to investigate effects of miR-1 on neural crest cell in the craniofacial development and its molecular mechanism. Methods: We knocked down miR-1 in zebrafish by miR-1 morpholino (MO) microinjection and observed phenotype of neural crest derivatives. We detected neural crest cell migration by time-lapse. Whole-mount in situ hybridization was used to monitor the expressions of genes involved in neural crest cell induction, specification, migration and differentiation. We performed a quantitative proteomics study (iTRAQ) and bioinformatics prediction to identify the targets of miR-1 and validate the relationship between miR-1 and its target gene sec63. Results: We found defects in the tissues derived from neural crest cells: a severely reduced lower jaw and delayed appearance of pigment cells. miR-1 MO injection also disrupted neural crest cell migration. At 24 hours post fertilization (hpf), reduced expression of tfap2a, dlx2, dlx3b, ngn1 and crestin indicated that miR-1 deficiency affected neural crest cell differentiation. iTRAQ and luciferase reporter assay identified SEC63 as a direct target gene of miR-1. The defects of miR-1 deficiency could be reversed, at least in part, by specific suppression of sec63 expression. Conclusion: miR-1 is involved in the regulation of neural crest cell development, and that it acts, at least partially, by targeting sec63 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping