PUBLICATION
Ishige okamurae Extract and Its Constituent Ishophloroglucin A Attenuated In Vitro and In Vivo High Glucose-Induced Angiogenesis
- Authors
- Fernando, K.H.N., Yang, H.W., Jiang, Y., Jeon, Y.J., Ryu, B.
- ID
- ZDB-PUB-191110-1
- Date
- 2019
- Source
- International Journal of Molecular Sciences 20(22): (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Ishige okamurae, Ishophloroglucin A, angiogenesis, diabetes
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Capillaries/drug effects
- Cell Line
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Glucose/toxicity*
- Humans
- Neovascularization, Pathologic/drug therapy*
- Phaeophyceae/chemistry*
- Phenols/pharmacology
- Phenols/therapeutic use*
- Plant Extracts/pharmacology
- Plant Extracts/therapeutic use*
- Polyphenols/pharmacology
- Polyphenols/therapeutic use*
- Signal Transduction/drug effects
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 31698871 Full text @ Int. J. Mol. Sci.
Citation
Fernando, K.H.N., Yang, H.W., Jiang, Y., Jeon, Y.J., Ryu, B. (2019) Ishige okamurae Extract and Its Constituent Ishophloroglucin A Attenuated In Vitro and In Vivo High Glucose-Induced Angiogenesis. International Journal of Molecular Sciences. 20(22):.
Abstract
Diabetes is associated with vascular complications, such as impaired wound healing and accelerated vascular growth. The different clinical manifestations, such as retinopathy and nephropathy, reveal the severity of enhanced vascular growth known as angiogenesis. This study was performed to evaluate the effects of an extract of Ishige okamurae (IO) and its constituent, Ishophloroglucin A (IPA) on high glucose-induced angiogenesis. A transgenic zebrafish (flk:EGFP) embryo model was used to evaluate vessel growth. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gap closure, transwell, and Matrigel® assays were used to analyze the proliferation, migration, and capillary formation of EA.hy926 cells. Moreover, protein expression were determined using western blotting. IO extract and IPA suppressed vessel formation in the transgenic zebrafish (flk:EGFP) embryo. IPA attenuated cell proliferation, cell migration, and capillary-like structure formation in high glucose-treated human vascular endothelial cells. Further, IPA down regulated the expression of high glucose-induced vascular endothelial growth factor receptor 2 (VEGFR-2) and downstream signaling molecule cascade. Overall, the IO extract and IPA exhibited anti-angiogenic effects against high glucose-induced angiogenesis, suggesting their potential for use as therapeutic agents in diabetes-related angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping