PUBLICATION

Morphogenetic control of zebrafish cardiac looping by Bmp signaling

Authors
Lombardo, V.A., Heise, M., Moghtadaei, M., Bornhorst, D., Männer, J., Abdelilah-Seyfried, S.
ID
ZDB-PUB-191022-23
Date
2019
Source
Development (Cambridge, England)   146(22): (Journal)
Registered Authors
Abdelilah-Seyfried, Salim
Keywords
BMP, Cardiac looping, Hemodynamics, Wnt, Zebrafish
MeSH Terms
  • Animals
  • Anisotropy
  • Bone Morphogenetic Proteins/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/metabolism
  • Heart/embryology*
  • Microscopy, Confocal
  • Morphogenesis
  • Myocytes, Cardiac/metabolism*
  • Organogenesis
  • Signal Transduction*
  • Torque
  • Transcription Factors/metabolism
  • Wnt Proteins/metabolism
  • Wnt Signaling Pathway
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism
PubMed
31628109 Full text @ Development
Abstract
Cardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically-labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire conical cell shapes, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, Bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of Bone morphogenetic protein signalling, myocardial cells at the superior wall of the atrioventricular canal maintain cuboidal cell shapes and S-shaped bending is impaired. This inventory of cellular rearrangements and cardiac looping regulation may also be relevant for understanding the etiology of human congenital heart defects.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes