PUBLICATION

Anti-inflammatory and Regulatory Effects of Huanglian Jiedu Decoction on Lipid Homeostasis and the TLR4/MyD88 Signaling Pathway in LPS-Induced Zebrafish

Authors
Zhou, J., Gu, X., Fan, X., Zhou, Y., Wang, H., Si, N., Yang, J., Bian, B., Zhao, H.
ID
ZDB-PUB-191017-7
Date
2019
Source
Frontiers in Physiology   10: 1241 (Journal)
Registered Authors
Keywords
Huanglian Jiedu decoction, TLR4/MyD88 signaling pathway, anti-inflammatory activity, lipidomic profile, lipopolysaccharide, zebrafish
MeSH Terms
none
PubMed
31616320 Full text @ Front. Physiol.
Abstract
Huanglian Jiedu decoction (HLJDD) has been used in the clinical treatment of inflammatory conditions. To clarify the mechanism of its comprehensive anti-inflammatory activities, the correlation between lipid homeostasis and the TLR4/MyD88 signaling pathway in zebrafish was established in the present study. In the lipopolysaccharide (LPS)-induced inflammation in zebrafish model, RT-PCR assays of five inflammatory cytokines and six targeted proteins were measured. Lipidomics analysis was conducted to identify potential lipid markers. HLJDD displayed strong efficacies, with a 61% anti-inflammatory rate at a concentration of 50 μg/mL. The activation of TLR4/MyD88 played an essential role in the inflammatory process. All protein indexes in the HLJDD group exhibited a tendency to reverse back to normal levels. Moreover, 79 potential pathological lipid biomarkers were identified. Compared with the model group, 61 therapeutic lipid biomarkers were detected in HLJDD group. Most perturbations of lipids were ameliorated by HLJDD, mainly through the glycerophospholipid metabolic pathway. In the visual network study, the corresponding lipoproteins such as PLA2, SGMS, and SMDP were observed as important intermediates between lipid homeostasis and the TLR4/MyD88 signaling pathway.
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    Citation
    Zhou, J., Gu, X., Fan, X., Zhou, Y., Wang, H., Si, N., Yang, J., Bian, B., Zhao, H. (2019) Anti-inflammatory and Regulatory Effects of Huanglian Jiedu Decoction on Lipid Homeostasis and the TLR4/MyD88 Signaling Pathway in LPS-Induced Zebrafish. Frontiers in Physiology. 10:1241.