PUBLICATION
Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
- Authors
- Dóró, É., Jacobs, S.H., Hammond, F.R., Schipper, H., Pieters, R.P., Carrington, M., Wiegertjes, G.F., Forlenza, M.
- ID
- ZDB-PUB-190925-7
- Date
- 2019
- Source
- eLIFE 8: (Journal)
- Registered Authors
- Schipper, Henk
- Keywords
- Trypanosoma carassii, host-pathogen interaction, infectious disease, microbiology, swimming behavior, zebrafish
- MeSH Terms
-
- Animals
- Blood/parasitology*
- Cell Adhesion*
- Disease Models, Animal
- Intravital Microscopy
- Locomotion*
- Spatio-Temporal Analysis
- Trypanosoma/physiology*
- Trypanosomiasis/parasitology*
- Zebrafish
- PubMed
- 31547905 Full text @ Elife
Citation
Dóró, É., Jacobs, S.H., Hammond, F.R., Schipper, H., Pieters, R.P., Carrington, M., Wiegertjes, G.F., Forlenza, M. (2019) Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms. eLIFE. 8:.
Abstract
Trypanosomes are important disease agents of humans, livestock and cold-blooded species, including fish. The cellular morphology of trypanosomes is central to their motility, adaptation to the host's environments and pathogenesis. However, visualizing the behaviour of trypanosomes resident in a live vertebrate host has remained unexplored. In this study, we describe an infection model of zebrafish (Danio rerio) with Trypanosoma carassii. By combining high spatio-temporal resolution microscopy with the transparency of live zebrafish, we describe in detail the swimming behaviour of trypanosomes in blood and tissues of a vertebrate host. Besides the conventional tumbling and directional swimming, T. carassii can change direction through a 'whip-like' motion or by swimming backward. Further, the posterior end can act as an anchoring site in vivo. To our knowledge, this is the first report of a vertebrate infection model that allows detailed imaging of trypanosome swimming behaviour in vivo in a natural host environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping