PUBLICATION
Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
- Authors
- Yeo, S., Yoon, C., Lien, C.L., Song, T.K., Shung, K.K.
- ID
- ZDB-PUB-190925-2
- Date
- 2019
- Source
- Sensors (Basel, Switzerland) 19(19): (Journal)
- Registered Authors
- Lien, Ching-Ling (Ellen)
- Keywords
- Nakagami imaging, high-frequency ultrasound, spectral Doppler, zebrafish
- MeSH Terms
-
- Animals
- Heart/physiology*
- Image Enhancement
- Regeneration/physiology*
- Ultrasonography/methods*
- Zebrafish
- PubMed
- 31546705 Full text @ Sensors (Basel)
Citation
Yeo, S., Yoon, C., Lien, C.L., Song, T.K., Shung, K.K. (2019) Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging. Sensors (Basel, Switzerland). 19(19):.
Abstract
This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping