PUBLICATION
Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish
- Authors
- Sanz-Morejón, A., García-Redondo, A.B., Reuter, H., Marques, I.J., Bates, T., Galardi-Castilla, M., Große, A., Manig, S., Langa, X., Ernst, A., Piragyte, I., Botos, M.A., González-Rosa, J.M., Ruiz-Ortega, M., Briones, A.M., Salaices, M., Englert, C., Mercader, N.
- ID
- ZDB-PUB-190801-25
- Date
- 2019
- Source
- Cell Reports 28: 1296-1306.e6 (Journal)
- Registered Authors
- Englert, Christoph, Gonzalez-Rosa, Juan Manuel, Marques, Ines, Mercader Huber, Nadia
- Keywords
- Wilms tumor 1b, fin regeneration, heart regeneration, macrophages, zebrafish
- Datasets
- GEO:GSE118509, GEO:GSE118457, GEO:GSE115381
- MeSH Terms
-
- Animal Fins/physiology*
- Animals
- Heart/physiology*
- Macrophages/cytology
- Macrophages/metabolism*
- Regeneration*
- WT1 Proteins/genetics
- WT1 Proteins/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 31365871 Full text @ Cell Rep.
Citation
Sanz-Morejón, A., García-Redondo, A.B., Reuter, H., Marques, I.J., Bates, T., Galardi-Castilla, M., Große, A., Manig, S., Langa, X., Ernst, A., Piragyte, I., Botos, M.A., González-Rosa, J.M., Ruiz-Ortega, M., Briones, A.M., Salaices, M., Englert, C., Mercader, N. (2019) Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Cell Reports. 28:1296-1306.e6.
Abstract
Organ regeneration is preceded by the recruitment of innate immune cells, which play an active role during repair and regrowth. Here, we studied macrophage subtypes during organ regeneration in the zebrafish, an animal model with a high regenerative capacity. We identified a macrophage subpopulation expressing Wilms tumor 1b (wt1b), which accumulates within regenerating tissues. This wt1b+ macrophage population exhibited an overall pro-regenerative gene expression profile and different migratory behavior compared to the remainder of the macrophages. Functional studies showed that wt1b regulates macrophage migration and retention at the injury area. Furthermore, wt1b-null mutant zebrafish presented signs of impaired macrophage differentiation, delayed fin growth upon caudal fin amputation, and reduced cardiomyocyte proliferation following cardiac injury that correlated with altered macrophage recruitment to the regenerating areas. We describe a pro-regenerative macrophage subtype in the zebrafish and a role for wt1b in organ regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping