ZFIN ID: ZDB-PUB-190717-3
A genetically encoded fluorescent sensor for in vivo imaging of GABA
Marvin, J.S., Shimoda, Y., Magloire, V., Leite, M., Kawashima, T., Jensen, T.P., Kolb, I., Knott, E.L., Novak, O., Podgorski, K., Leidenheimer, N.J., Rusakov, D.A., Ahrens, M.B., Kullmann, D.M., Looger, L.L.
Date: 2019
Source: Nature Methods   16(8): 763-770 (Journal)
Registered Authors: Ahrens, Misha, Kawashima, Takashi
Keywords: none
MeSH Terms:
  • Anesthesia
  • Animals
  • Animals, Genetically Modified
  • Biosensing Techniques/methods*
  • Brain/metabolism*
  • Female
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism*
  • Hippocampus/metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Imaging/methods*
  • Neurons/metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Seizures/metabolism
  • Seizures/pathology
  • Zebrafish
  • gamma-Aminobutyric Acid/metabolism*
PubMed: 31308547 Full text @ Nat. Methods
Current techniques for monitoring GABA (γ-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.