PUBLICATION
Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species
- Authors
- Wu, S.H., Chou, H.Y., Liu, P.C., Wu, J.L., Gong, H.Y.
- ID
- ZDB-PUB-190612-2
- Date
- 2019
- Source
- Biochemical and Biophysical Research Communications 515(4): 706-711 (Journal)
- Registered Authors
- Gong, Hong-Yi, Wu, Jen-Leih
- Keywords
- Antimicrobial peptide, Bactericidal, Bacteriostatic, Granulin, Tilapia, Vibrio
- MeSH Terms
-
- Alternative Splicing
- Animals
- Anti-Bacterial Agents/pharmacology*
- Antimicrobial Cationic Peptides/pharmacology*
- Granulins
- Hemolysis
- Hydrogen-Ion Concentration
- Microscopy, Electron, Transmission
- Sheep
- Temperature
- Tilapia/metabolism*
- Vibrio/drug effects*
- Vibrio Infections/drug therapy*
- PubMed
- 31182280 Full text @ Biochem. Biophys. Res. Commun.
Citation
Wu, S.H., Chou, H.Y., Liu, P.C., Wu, J.L., Gong, H.Y. (2019) Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species. Biochemical and Biophysical Research Communications. 515(4):706-711.
Abstract
In our previous study, the novel GRN-41 peptide generated from alternative splicing of the Mozambique tilapia PGRN1 gene was identified to be a potent peptide that protected against V. vulnificus in the transgenic zebrafish model by modulating innate immune-related genes. In this study, the anti-bacterial activities of synthetic Mozambique tilapia GRN-41 peptide (OmGRN-41) against various bacterial pathogens were investigated. The results showed that OmGRN-41 had bactericidal activity against Vibrio species, including V. vulnificus, V. alginolyticus, and V. harveyi, but exhibited bacteriostatic activity against V. parahaemolyticus. OmGRN-41 maintained bactericidal activity (64 μM) against V. vulnificus at pH 2 to pH 10 or after heat treatment for 1 h at high temperatures between 40 °C and 100 °C. TEM observations revealed that the outer membrane of V. vulnificus was disrupted by OmGRN-41, leading to morphological rupture and loss of cytoplasmic contents. Additionally, little hemolytic activity against tilapia and sheep erythrocytes was detected after treatment with 128 μM OmGRN-41. OmGRN-41 can effectively enhance the survival of Nile tilapia infected by V. vulnificus. Our results suggest that the OmGRN-41 is a novel antimicrobial peptide possessing bactericidal activity, especially against Vibrio species. These results indicate that OmGRN-41 can be applied in human Vibriosis treatment and has the potential to defend against Vibrio spp. infection in critical aquaculture organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping