PUBLICATION
Studies Into β-Glucan Recognition in Fish Suggests a Key Role for the C-Type Lectin Pathway
- Authors
- Petit, J., Bailey, E.C., Wheeler, R.T., de Oliveira, C.A.F., Forlenza, M., Wiegertjes, G.F.
- ID
- ZDB-PUB-190314-10
- Date
- 2019
- Source
- Frontiers in immunology 10: 280 (Journal)
- Registered Authors
- Wheeler, Robert
- Keywords
- C-type lectin-like domain, CTLD, Cyprinidae, RNAseq analysis, primary macrophage, teleost, transcriptome analysis, β-glucan
- MeSH Terms
-
- Animals
- Carps/genetics
- Carps/immunology*
- Carps/metabolism
- Cells, Cultured
- Fish Proteins/genetics
- Fish Proteins/immunology*
- Fish Proteins/metabolism
- Gene Expression Profiling/methods
- Gene Ontology
- Lectins, C-Type/classification
- Lectins, C-Type/genetics
- Lectins, C-Type/immunology*
- Macrophages/immunology*
- Macrophages/metabolism
- Phylogeny
- Signal Transduction/genetics
- Signal Transduction/immunology
- Synteny/genetics
- Synteny/immunology
- Transcriptome/genetics
- Transcriptome/immunology*
- Zebrafish/genetics
- Zebrafish/immunology
- Zebrafish/metabolism
- beta-Glucans/immunology*
- beta-Glucans/metabolism
- PubMed
- 30863400 Full text @ Front Immunol
Citation
Petit, J., Bailey, E.C., Wheeler, R.T., de Oliveira, C.A.F., Forlenza, M., Wiegertjes, G.F. (2019) Studies Into β-Glucan Recognition in Fish Suggests a Key Role for the C-Type Lectin Pathway. Frontiers in immunology. 10:280.
Abstract
Immune-modulatory effects of β-glucans are generally considered beneficial to fish health. Despite the frequent application of β-glucans in aquaculture practice, the exact receptors and downstream signalling remains to be described for fish. In mammals, Dectin-1 is a member of the C-type lectin receptor (CLR) family and the best-described receptor for β-glucans. In fish genomes, no clear homologue of Dectin-1 could be identified so far. Yet, in previous studies we could activate carp macrophages with curdlan, considered a Dectin-1-specific β-(1,3)-glucan ligand in mammals. It was therefore proposed that immune-modulatory effects of β-glucan in carp macrophages could be triggered by a member of the CLR family activating the classical CLR signalling pathway, different from Dectin-1. In the current study, we used primary macrophages of common carp to examine immune modulation by β-glucans using transcriptome analysis of RNA isolated 6 h after stimulation with two different β-glucan preparations. Pathway analysis of differentially expressed genes (DEGs) showed that both β-glucans regulate a comparable signalling pathway typical of CLR activation. Carp genome analysis identified 239 genes encoding for proteins with at least one C-type Lectin Domains (CTLD). Narrowing the search for candidate β-glucan receptors, based on the presence of a conserved glucan-binding motif, identified 13 genes encoding a WxH sugar-binding motif in their CTLD. These genes, however, were not expressed in macrophages. Instead, among the β-glucan-stimulated DEGs, a total of six CTLD-encoding genes were significantly regulated, all of which were down-regulated in carp macrophages. Several candidates had a protein architecture similar to Dectin-1, therefore potential conservation of synteny of the mammalian Dectin-1 region was investigated by mining the zebrafish genome. Partial conservation of synteny with a region on the zebrafish chromosome 16 highlighted two genes as candidate β-glucan receptor. Altogether, the regulation of a gene expression profile typical of a signalling pathway associated with CLR activation and, the identification of several candidate β-glucan receptors, suggest that immune-modulatory effects of β-glucan in carp macrophages could be a result of signalling mediated by a member of the CLR family.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping