PUBLICATION

Expression of adiponectin receptors in the brain of adult zebrafish and mouse: Links with neurogenic niches and brain repair

Authors
Rastegar, S., Parimisetty, A., Cassam-Sulliman, N., Narra Sai, S., Weber, S., Rastegar, M., Viranaicken, W., Couret, D., Planesse, C., Strähle, U., Meilhac, O., Lefebvre d'Hellencourt, C., Diotel, N.
ID
ZDB-PUB-190308-16
Date
2019
Source
The Journal of comparative neurology   527(14): 2317-2333 (Journal)
Registered Authors
Diotel, Nicolas, Rastegar, Maryam, Rastegar, Sepand, Strähle, Uwe, Weber, Sabrina
Keywords
RRID: AB_10013383, RRID: AB_10049650, RRID: AB_141372, RRID: AB_2160651, RRID: AB_221448, RRID: AB_2314535, RRID: AB_2534069, RRID: AB_514497, RRID: AB_514499, RRID: SCR_003070, adipor, brain ischemia, brain repair, neural stem cells, stab wound
MeSH Terms
  • Age Factors
  • Animals
  • Brain/cytology
  • Brain/metabolism*
  • Brain Chemistry/physiology
  • Gene Expression
  • Inflammation/genetics
  • Inflammation/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neural Stem Cells/chemistry
  • Neural Stem Cells/metabolism*
  • Neurogenesis/physiology*
  • Receptors, Adiponectin/analysis
  • Receptors, Adiponectin/biosynthesis*
  • Receptors, Adiponectin/genetics
  • Species Specificity
  • Zebrafish
PubMed
30843204 Full text @ J. Comp. Neurol.
Abstract
Adiponectin and its receptors (adipor) have been initially characterized for their role in lipid and glucose metabolism. More recently, adiponectin signaling was shown to display anti-inflammatory effects and to participate in brain homeostasis and neuroprotection. In this study, we investigated adipor gene expression and its regulation under inflammatory conditions in two complementary models: mouse and zebrafish. We demonstrate that adipor1a, adipor1b and adipor2 are widely distributed throughout the brain of adult fish, in neurons and also in radial glia, behaving as neural stem cells. We also show that telencephalic injury results in a decrease in adipor gene expression, inhibited by an anti-inflammatory treatment (Dexamethasone). Interestingly, adiponectin injection after brain injury led to a consistent decrease (1) in the recruitment of microglial cells at the lesioned site and (2) in the proliferation of neural progenitors, arguing for a neuroprotective role of adiponectin. In a comparative approach, we investigate Adipor1 and Adipor2 gene distribution in the brain of mice and demonstrated their expression in regions shared with fish including neurogenic regions. We also document Adipor gene expression in mice after middle cerebral artery occlusion (MCAO) and LPS injection. In contrast to zebrafish, these inflammatory stimuli do no impact cerebral adiponectin receptor gene expression in mouse. This work provides new insights regarding adipor expression in the brain of fish, and demonstrates evolutionary conserved distribution of adipor with mouse. This is the first report of adipor expression in adult neural stem cells of fish, suggesting a potential role of adiponectin signaling during vertebrate neurogenesis. It also suggests a potential contribution of inflammation in the regulation of adipor in fish. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes