PUBLICATION
Human macrophages survive and adopt activated genotypes in living zebrafish
- Authors
- Paul, C.D., Devine, A., Bishop, K., Xu, Q., Wulftange, W.J., Burr, H., Daly, K.M., Lewis, C., Green, D.S., Staunton, J.R., Choksi, S., Liu, Z.G., Sood, R., Tanner, K.
- ID
- ZDB-PUB-190212-5
- Date
- 2019
- Source
- Scientific Reports 9: 1759 (Journal)
- Registered Authors
- Greenspan, Daniel S., Sood, Raman
- Keywords
- none
- MeSH Terms
-
- Humans
- Zebrafish
- Macrophage Activation/genetics*
- Macrophage Activation/immunology*
- Animals
- Phenotype
- Genotype*
- Cell Survival/genetics
- Cell Survival/immunology
- Macrophages/immunology*
- Macrophages/metabolism*
- Biomarkers
- PubMed
- 30741975 Full text @ Sci. Rep.
Citation
Paul, C.D., Devine, A., Bishop, K., Xu, Q., Wulftange, W.J., Burr, H., Daly, K.M., Lewis, C., Green, D.S., Staunton, J.R., Choksi, S., Liu, Z.G., Sood, R., Tanner, K. (2019) Human macrophages survive and adopt activated genotypes in living zebrafish. Scientific Reports. 9:1759.
Abstract
The inflammatory response, modulated both by tissue resident macrophages and recruited monocytes from peripheral blood, plays a critical role in human diseases such as cancer and neurodegenerative disorders. Here, we sought a model to interrogate human immune behavior in vivo. We determined that primary human monocytes and macrophages survive in zebrafish for up to two weeks. Flow cytometry revealed that human monocytes cultured at the physiological temperature of the zebrafish survive and differentiate comparable to cohorts cultured at human physiological temperature. Moreover, key genes that encode for proteins that play a role in tissue remodeling were also expressed. Human cells migrated within multiple tissues at speeds comparable to zebrafish macrophages. Analysis of gene expression of in vivo educated human macrophages confirmed expression of activated macrophage phenotypes. Here, human cells adopted phenotypes relevant to cancer progression, suggesting that we can define the real time immune modulation of human tumor cells during the establishment of a metastatic lesion in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping