PUBLICATION
In vivo pro-angiogenic effects of dracorhodin perchlorate in zebrafish embryos: A novel bioactivity evaluation platform for commercial dragon blood samples
- Authors
- Krishnaraj, P., Chang, Y., Ho, T.J., Lu, N.C., Lin, M.D., Chen, H.P.
- ID
- ZDB-PUB-190117-2
- Date
- 2019
- Source
- Journal of food and drug analysis 27: 259-265 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Dracorhodin chlorate, Dragon blood
- MeSH Terms
-
- Angiogenesis Inducing Agents/analysis
- Angiogenesis Inducing Agents/pharmacology*
- Animals
- Benzopyrans/analysis
- Benzopyrans/pharmacology*
- Cell Line, Tumor
- Chromatography, High Pressure Liquid
- Drug Evaluation, Preclinical/methods*
- Plant Extracts/analysis
- Plant Extracts/pharmacology*
- Zebrafish/embryology
- PubMed
- 30648579 Full text @ J Food Drug Anal
Citation
Krishnaraj, P., Chang, Y., Ho, T.J., Lu, N.C., Lin, M.D., Chen, H.P. (2019) In vivo pro-angiogenic effects of dracorhodin perchlorate in zebrafish embryos: A novel bioactivity evaluation platform for commercial dragon blood samples. Journal of food and drug analysis. 27:259-265.
Abstract
Dragon blood has been used in wound treatment for many years and can be obtained from several distinct plant species. Dracorhodin, the active substituent of dragon blood, is a characteristic compound of the palm tree, Daemonorops draco. At present, the only method to evaluate the quality of commercial dragon blood samples is a HPLC method which determines the amount of dracorhodin in a dragon blood sample. In this study, we used zebrafish embryos as a platform to demonstrate the in vivo pro-angiogenic activity of dracorhodin perchlorate, the chemically synthesized analog of dracorhodin. By using this platform, three different commercial dragon blood samples were also examined. Our results clearly show that even though the commercial dragon blood samples had similar amounts of dracorhodin, they showed highly variable biological activity, such as pro-angiogenic effects and toxicity. In short, an in vivo activity assay platform for rapidly examining the biological activity of commercial dragon blood samples was successfully established here, which complements the current HPLC-based assay method.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping