PUBLICATION

Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae

Authors
Zeng, Z., Lopez-Baez, J.C., Lleras-Forero, L., Brunsdon, H., Wyatt, C., Rybski, W., Hastie, N.D., Schulte-Merker, S., Patton, E.E.
ID
ZDB-PUB-190101-3
Date
2018
Source
Bio-protocol   8: e3100 (Journal)
Registered Authors
Hastie, Nicholas D., Patton, E. Elizabeth, Schulte-Merker, Stefan, Wyatt, Cameron
Keywords
Amputation, Injury, Notochord, Nystatin, Tail fin, Tissue repair, Tungsten wire, Zebrafish
MeSH Terms
none
PubMed
30596125 Full text @ Bio Protoc
Abstract
Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific transcriptional responses in notochord cellular subpopulations. The notochord is a critical embryonic structure that functions to shape and pattern the vertebrae and spinal column. Here, we describe precision needle injury, tail-notochord amputation, and chemical inhibition of caveolin that trigger a wound-specific wt1b expression response in the notochord sheath cell subpopulation. We propose that these procedures can be used to study distinct cell populations that make up the cellular processes of notochord repair.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes