PUBLICATION

Folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks: fluorescence and magnetic resonance dual-modality imaging and photodynamic therapy in hepatocellular carcinoma

Authors
Chen, Y., Liu, W., Shang, Y., Cao, P., Cui, J., Li, Z., Yin, X., Li, Y.
ID
ZDB-PUB-181228-5
Date
2018
Source
International Journal of Nanomedicine   14: 57-74 (Journal)
Registered Authors
Keywords
dual-modality imaging, folic acid, hepatocellular carcinoma, nanoscale gadolinium-porphyrin metal-organic frameworks, photodynamic therapy
MeSH Terms
  • Folic Acid/chemistry*
  • Folic Acid/therapeutic use*
  • Carcinoma, Hepatocellular/drug therapy*
  • Carcinoma, Hepatocellular/pathology
  • Zebrafish/embryology
  • Embryo, Nonmammalian/drug effects
  • Time-Lapse Imaging
  • Hep G2 Cells
  • Humans
  • Fluorescence
  • Photochemotherapy*
  • Animals
  • Cell Death
  • Magnetic Resonance Imaging*
  • Gadolinium/chemistry*
  • Liver Neoplasms/drug therapy*
  • Liver Neoplasms/pathology
  • Nanoparticles/chemistry
  • Nanoparticles/ultrastructure
  • Cell Survival/drug effects
  • Metal-Organic Frameworks/chemical synthesis
  • Metal-Organic Frameworks/chemistry*
(all 22)
PubMed
30587985 Full text @ Int. J. Nanomedicine
Abstract
Hepatocellular carcinoma (HCC) is the most common primary liver cancer and severely threatens human health. Since the prognosis of advanced HCC remains poor, there is an urgent need to develop new therapeutic approaches. Porphyrin metal-organic frameworks are a class of porous organic-inorganic hybrid functional materials with good biocompatibility.
Gadolinium-porphyrin metal-organic frameworks were used as a skeleton for folic acid (FA) conjugation to synthesize a novel type of nanoparticle, denoted as folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks (FA-NPMOFs). The FA-NPMOFs were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric-differential thermal analysis. The biotoxicity and imaging capability of the FA-NPMOFs were determined using HepG2 cells and embryonic and larval zebrafish. The delivery and photodynamic therapeutic effect of FA-NPMOFs were explored in transgenic zebrafish with doxycycline-induced HCC.
FA-NPMOFs were spherical in structure with good dispersion and water solubility. They showed low biotoxicity, emitted bright red fluorescence, and exhibited an excellent magnetic resonance imaging capability, both in vitro and in vivo. Meanwhile, the FA-NPMOFs exhibited a strong affinity for folate receptor (FR)-expressing cells and were delivered to the tumor site in a targeted manner. Moreover, HCC tumor cells were eliminated following laser irradiation.
FA-NPMOFs can be used for dual-modality imaging and photodynamic therapy in HCC and show promise for use as a carrier in new therapies for HCC and other FR-positive tumors.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gz32TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-folrmonoclonal
      IgGMouse
      1 - 1 of 1
      Show
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
      Show
      Mapping
      No data available