PUBLICATION

Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development

Authors
Lu, C.J., Fan, X.Y., Guo, Y.F., Cheng, Z.C., Dong, J., Chen, J.Z., Li, L.Y., Wang, M.W., Wu, Z.K., Wang, F., Tong, X.J., Luo, L.F., Tang, F.C., Zhu, Z.Y., Zhang, B.
ID
ZDB-PUB-181109-6
Date
2018
Source
Journal of molecular cell biology   11(6): 435-447 (Journal)
Registered Authors
Zhang, Bo
Keywords
none
MeSH Terms
  • Islets of Langerhans/embryology*
  • Transcription, Genetic*
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Single-Cell Analysis*
  • Animals
  • Antigens, Differentiation/biosynthesis*
  • Antigens, Differentiation/genetics
  • Gene Expression Regulation, Developmental*
(all 11)
PubMed
30407522 Full text @ J. Mol. Cell Biol.
Abstract
Pancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy. We identified proliferative endocrine progenitors, which could be further categorized by different cell cycle phases with the G1/S sub-population displaying a distinct differentiation potential. We identified endocrine precursors, a heterogeneous intermediate-state population consisting of lineage-primed alpha, beta and delta cells that were characterized by the expression of lineage-specific transcription factors and relatively low expression of terminally differentiation markers. The terminally differentiated alpha, beta and delta cells displayed stage-dependent differentiation states, which were related to their functional maturation. Our data unveiled distinct states, events and molecular features during the islet developmental transition, and provided resources to comprehensively understand the lineage hierarchy of islet development at the single-cell level.
Genes / Markers
Figures
Figure Gallery (3 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nl1TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    tbx2bMO8-tbx2bMRPHLNO
    1 - 1 of 1
    Show
    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab8-mapk14polyclonal
      		IgGRabbit
      1 - 1 of 1
      Show
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
      Show
      Mapping
      No data available
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Lu, C.J., Fan, X.Y., Guo, Y.F., Cheng, Z.C., Dong, J., Chen, J.Z., Li, L.Y., Wang, M.W., Wu, Z.K., Wang, F., Tong, X.J., Luo, L.F., Tang, F.C., Zhu, Z.Y., Zhang, B. (2018) Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development. Journal of molecular cell biology. 11(6):435-447.