PUBLICATION
Fertility Enhancement but Premature Ovarian Failure in esr1-Deficient Female Zebrafish.
- Authors
- Chen, Y., Tang, H., Wang, L., He, J., Guo, Y., Liu, Y., Liu, X., Lin, H.
- ID
- ZDB-PUB-181016-10
- Date
- 2018
- Source
- Frontiers in endocrinology 9: 567 (Journal)
- Registered Authors
- Liu, Xiaochun
- Keywords
- POF, esr1, fertility, mTOR pathway, zebrafish
- MeSH Terms
- none
- PubMed
- 30319547 Full text @ Front Endocrinol (Lausanne)
Citation
Chen, Y., Tang, H., Wang, L., He, J., Guo, Y., Liu, Y., Liu, X., Lin, H. (2018) Fertility Enhancement but Premature Ovarian Failure in esr1-Deficient Female Zebrafish.. Frontiers in endocrinology. 9:567.
Abstract
It is well established that estrogens regulate female reproduction through estrogen receptors (ERs) in the ovary. However, the precise physiological role of estrogen/ER signaling in reproduction processes remains poorly defined in zebrafish. In this study, we successfully generated an ERα (esr1) mutant line in zebrafish via transcription activator-like effectors nucleases (TALENs). It was found in the mutant females that the fertility was enhanced and the ovarian histology was normal at 90 days post-fertilization (dpf). However, the number of fertile females decreased with age. By 180 dpf, esr1 mutant females were infertile with degenerated ovaries, while the age-matched wild-type females were still fertile. Additionally, few large vitellogenic granules can be found in full grown (FG) follicles at 90 dpf and the expression of vtg genes were down-regulated at both 90 and 180 dpf in esr1 mutant zebrafish. Moreover, steroidogenesis pathway and mTOR signaling pathway were over-activated at 90 dpf, but declined prematurely in esr1 mutant zebrafish by 180 dpf. Collectively, the present study provides evidence that esr1 is fundamental for ovarian maintenance in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping