PUBLICATION
Reversible deafferentation of the zebrafish olfactory bulb with wax pluginsertion
- Authors
- Scheib, J.J., Pozzuto, J.M., Byrd-Jacobs, C.A.
- ID
- ZDB-PUB-181015-2
- Date
- 2018
- Source
- Journal of Neuroscience Methods 311: 47-56 (Journal)
- Registered Authors
- Byrd-Jacobs, Christine
- Keywords
- deafferentation, naris occlusion, olfactory bulb, plasticity, reinnervation, zebrafish
- MeSH Terms
-
- Animals
- Female
- Male
- Models, Animal*
- Nasal Cavity/surgery*
- Nerve Regeneration
- Olfactory Bulb/cytology
- Olfactory Bulb/physiology*
- Olfactory Receptor Neurons/cytology
- Olfactory Receptor Neurons/physiology*
- Waxes
- Zebrafish/physiology*
- PubMed
- 30316892 Full text @ J. Neurosci. Methods
Citation
Scheib, J.J., Pozzuto, J.M., Byrd-Jacobs, C.A. (2018) Reversible deafferentation of the zebrafish olfactory bulb with wax pluginsertion. Journal of Neuroscience Methods. 311:47-56.
Abstract
Background Deafferentation of the zebrafish olfactory bulb allows investigation of neuroplasticity in a particularly dynamic brain region of a popular model animal known for its regenerative abilities. Current methods to remove sensory input to the zebrafish olfactory bulb differ in the extent of deafferentation and potential for recovery.
New method We present a novel method of olfactory bulb deafferentation using continuous wax plug insertions into the nasal cavity of zebrafish. Wax plugs were placed in the nasal cavity and replaced if needed over 1wk or 3wk survival periods. Wax plugs were removed from fish after 1wk of occlusion to analyze the potential recovery of the olfactory organ and bulb.
Results Wax plug insertions caused a dramatic reduction in olfactory organ size and structure and significantly reduced afferent input to the olfactory bulb after 1wk and 3wk. Removal of the wax plugs after 1wk allowed for recovery of the olfactory organ and subsequent reinnervation of the olfactory bulb.
Comparisons with existing methods Chemical ablation with detergent causes partial, temporary deafferentation of the olfactory bulb. Cautery ablation causes complete, permanent deafferentation of the olfactory bulb. Wax plug insertions cause nearly complete, temporary deafferentation, allowing both significant deafferentation and the potential for reinnervation of the olfactory bulb.
Conclusions The wax plug insertion method of deafferentation described here is unique in that it destroys almost completely the structure of the olfactory organ and removes almost completely sensory input to the olfactory bulb, yet the organ returns to its typical morphology and afferent innervation returns.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping