PUBLICATION
Deafferentation-induced alterations in mitral cell dendritic morphology in the adult zebrafish olfactory bulb
- Authors
- Pozzuto, J.M., Fuller, C.L., Byrd-Jacobs, C.A.
- ID
- ZDB-PUB-180915-4
- Date
- 2018
- Source
- Journal of bioenergetics and biomembranes 51(1): 29-40 (Journal)
- Registered Authors
- Byrd-Jacobs, Christine
- Keywords
- Dextran, Modified Sholl analysis, Output neurons, Retrograde labeling, Teleosts
- MeSH Terms
-
- Animals
- Dendrites/ultrastructure*
- Neuronal Plasticity/physiology
- Neurons, Afferent/physiology*
- Olfactory Bulb/cytology
- Olfactory Bulb/physiology*
- Olfactory Bulb/ultrastructure
- Zebrafish
- PubMed
- 30215151 Full text @ J. Bioenerg. Biomembr.
Citation
Pozzuto, J.M., Fuller, C.L., Byrd-Jacobs, C.A. (2018) Deafferentation-induced alterations in mitral cell dendritic morphology in the adult zebrafish olfactory bulb. Journal of bioenergetics and biomembranes. 51(1):29-40.
Abstract
The removal of afferent input to the olfactory bulb by both cautery and chemical olfactory organ ablation in adult zebrafish results in a significant decrease in volume of the ipsilateral olfactory bulb. To examine the effects of deafferentation at a cellular level, primary output neurons of the olfactory bulb, the mitral cells, were investigated using retrograde tract tracing with fluorescent dextran using ex vivo brain cultures. Morphological characteristics including the number of major dendritic branches, total length of dendritic branches, area of the dendritic arbor, overall dendritic complexity, and optical density of the arbor were used to determine the effects of deafferentation on mitral cell dendrites. Following 8 weeks of permanent deafferentation there were significant reductions in the total length of dendritic branches, the area of the dendritic arbor, and the density of fine processes in the dendritic tuft. With 8 weeks of chronic, partial deafferentation there were significant reductions in all parameters examined, including a modified Sholl analysis that showed significant decreases in overall dendritic complexity. These results show the plasticity of mitral cell dendritic structures in the adult brain and provide information about the response of these output neurons following the loss of sensory input in this key model system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping