PUBLICATION
Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography
- Authors
- Lapierre-Landry, M., Huckenpahler, A.L., Link, B.A., Collery, R.F., Carroll, J., Skala, M.C.
- ID
- ZDB-PUB-180913-2
- Date
- 2018
- Source
- Translational vision science & technology 7: 4 (Journal)
- Registered Authors
- Collery, Ross, Link, Brian
- Keywords
- melanin, optical coherence tomography, retinal pigment epithelium, zebrafish
- MeSH Terms
- none
- PubMed
- 30197836 Full text @ Transl Vis Sci Technol
Citation
Lapierre-Landry, M., Huckenpahler, A.L., Link, B.A., Collery, R.F., Carroll, J., Skala, M.C. (2018) Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography. Translational vision science & technology. 7:4.
Abstract
Purpose To demonstrate and validate that photothermal optical coherence tomography (PT-OCT) can image melanin in the retinal pigment epithelium (RPE) and can observe light-driven melanosome translocation in the zebrafish retina.
Methods A commercial spectral domain OCT system was modified to perform both OCT and PT-OCT. Four adult tyrosinase-mosaic zebrafish with varying levels of melanin expression across their retinas were imaged, and the PT-OCT signal for pigmented and nonpigmented regions were compared. Wild-type dark-adapted (n = 11 fish) and light-adapted (n = 10 fish) zebrafish were also imaged with OCT and PT-OCT. Longitudinal reflectivity and absorption profiles were generated from B-scans to compare the melanin distribution between the two groups.
Results A significant increase in PT-OCT signal (P < 0.0001, Student's t-test) was observed in pigmented regions of interest (ROI) compared to nonpigmented ROIs in the tyrosinase-mosaic zebrafish, which confirms the PT-OCT signal is specific to melanin in the eye. A significant increase in PT-OCT signal intensity (P < 0.0001, Student's t-test) was also detected in the light-adapted wild-type zebrafish group compared to the dark-adapted group. Additionally, light-adapted zebrafish display more distinct melanin banding patterns than do dark-adapted zebrafish in PT-OCT B-scans.
Conclusions PT-OCT can detect different levels of melanin absorption and characterize pigment distribution in the zebrafish retina, including intracellular changes due to light-driven melanosome translocation within the RPE.
Translational relevance PT-OCT could quantify changes in pigmentation that occur in retinal diseases. The functional information provided by PT-OCT may also enable a better understanding of the anatomical features within conventional OCT images.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping