PUBLICATION
The mitochondrial transporter SLC25A25 links ciliary TRPP2 signaling and cellular metabolism
- Authors
- Hofherr, A., Seger, C., Fitzpatrick, F., Busch, T., Michel, E., Luan, J., Osterried, L., Linden, F., Kramer-Zucker, A., Wakimoto, B., Schütze, C., Wiedemann, N., Artati, A., Adamski, J., Walz, G., Kunji, E.R.S., Montell, C., Watnick, T., Köttgen, M.
- ID
- ZDB-PUB-180807-6
- Date
- 2018
- Source
- PLoS Biology 16: e2005651 (Journal)
- Registered Authors
- Kramer-Zucker, Albrecht, Seger, Claudia
- Keywords
- none
- MeSH Terms
-
- Amino Acid Transport Systems, Acidic/metabolism*
- Animals
- Antiporters/metabolism
- Calcium/metabolism
- Calcium Channels/metabolism
- Calcium-Binding Proteins/metabolism*
- Cilia/metabolism*
- Drosophila melanogaster/metabolism
- Heterozygote
- Humans
- Ion Channels/metabolism
- Mitochondrial Membrane Transport Proteins/metabolism
- Mitochondrial Proteins/metabolism
- Signal Transduction
- TRPP Cation Channels/metabolism*
- Zebrafish
- PubMed
- 30080851 Full text @ PLoS Biol.
Citation
Hofherr, A., Seger, C., Fitzpatrick, F., Busch, T., Michel, E., Luan, J., Osterried, L., Linden, F., Kramer-Zucker, A., Wakimoto, B., Schütze, C., Wiedemann, N., Artati, A., Adamski, J., Walz, G., Kunji, E.R.S., Montell, C., Watnick, T., Köttgen, M. (2018) The mitochondrial transporter SLC25A25 links ciliary TRPP2 signaling and cellular metabolism. PLoS Biology. 16:e2005651.
Abstract
Cilia are organelles specialized in movement and signal transduction. The ciliary transient receptor potential ion channel polycystin-2 (TRPP2) controls elementary cilia-mediated physiological functions ranging from male fertility and kidney development to left-right patterning. However, the molecular components translating TRPP2 channel-mediated Ca2+ signals into respective physiological functions are unknown. Here, we show that the Ca2+-regulated mitochondrial ATP-Mg/Pi solute carrier 25 A 25 (SLC25A25) acts downstream of TRPP2 in an evolutionarily conserved metabolic signaling pathway. We identify SLC25A25 as an essential component in this cilia-dependent pathway using a genome-wide forward genetic screen in Drosophila melanogaster, followed by a targeted analysis of SLC25A25 function in zebrafish left-right patterning. Our data suggest that TRPP2 ion channels regulate mitochondrial SLC25A25 transporters via Ca2+ establishing an evolutionarily conserved molecular link between ciliary signaling and mitochondrial metabolism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping