|ZFIN ID: ZDB-PUB-180727-9|
Nuclear localization of Hif-3α requires two redundant NLS motifs in its unique C-terminal region
Yao, Q., Zhang, P., Lu, L., Liu, Y., Li, Y., Duan, C.
|Source:||FEBS letters 592(16): 2769-2775 (Journal)|
|Registered Authors:||Duan, Cunming, Liu, Yunzhang|
|Keywords:||Hypoxia, hypoxia-inducible factor, nuclear localization signal, zebrafish|
|PubMed:||30047986 Full text @ FEBS Lett.|
Yao, Q., Zhang, P., Lu, L., Liu, Y., Li, Y., Duan, C. (2018) Nuclear localization of Hif-3α requires two redundant NLS motifs in its unique C-terminal region. FEBS letters. 592(16):2769-2775.
ABSTRACTHif-3α, a member of the hypoxia-inducible factor (HIF) family, enters the nucleus and regulates gene expression in response to hypoxia. The molecular basis of its nuclear localization is not clear. HIF-1α and HIF-2α use a bipartite nuclear localization signal (NLS) to enter the nucleus. This motif is not conserved in Hif-3α. Although there is a conserved Arg/Lys rich motif in the Hif-3α N-terminal region, deletion of this region has minimal effect on Hif-3α nuclear localization. Here, we mapped the functional NLS to the unique C-terminal region of Hif-3α and identified 2 clusters of basic residues critical for its nuclear localization. The two NLS motifs are functionally redundant. Our results, thus, suggest that Hif-3α nuclear localization is mediated through two redundant NLS motifs located in its unique C-terminal region. This article is protected by copyright. All rights reserved.
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