|ZFIN ID: ZDB-PERS-960805-152|
BIOGRAPHY AND RESEARCH INTERESTS
Dr. Duan is interested in the physiological functions of peptide growth factors and the molecular mechanisms by which they act to control animal development and growth. His current research focuses on the insulin-like growth factors, their receptors and binding proteins. Dr. Duan's research makes use of whole animal models, as well as cell culture systems. These include both mammalian and teleost fish systems. A combination of molecular, cellular, biochemical and comparative approaches is used to address physiological and evolutionary questions. Current research programs in the laboratory include: 1) the role of IGFs, their receptors and binding proteins in regulating animal growth and development; 2) molecular and cellular mechanisms by which IGFs act to control cell proliferation, differentiation and migration and 3) structural and functional conservation of insulin/IGF superfamily peptides and related molecules throughout vertebrate evolution.
Liu, C., Li, S., Noer, P.R., Kjaer-Sorensen, K., Juhl, A.K., Goldstein, A., Ke, C., Oxvig, C., Duan, C. (2020) The metalloproteinase Papp-aa controls epithelial cell quiescence-proliferation transition. eLIFE. 9:
Duan, C., Allard, J. (2020) Gonadotropin-releasing hormone neuron development in vertebrates. General and comparative endocrinology. 292:113465
Xin, Y., Malick, A., Hu, M., Liu, C., Batah, H., Xu, H., Duan, C. (2019) Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6. eLIFE. 8:
Liu, C., Xin, Y., Bai, Y., Lewin, G., He, G., Mai, K., Duan, C. (2018) Ca2+ concentration-dependent premature death of igfbp5a-/- fish reveals a critical role of IGF signaling in adaptive epithelial growth.. Science signaling. 11(548):
Yao, Q., Zhang, P., Lu, L., Liu, Y., Li, Y., Duan, C. (2018) Nuclear localization of Hif-3α requires two redundant NLS motifs in its unique C-terminal region. FEBS letters. 592(16):2769-2775
Allard, J.B., and Duan, C. (2018) IGF-Binding Proteins: Why Do They Exist and Why Are There So Many?. Frontiers in endocrinology. 9:117
Kamei, H., Yoneyama, Y., Hakuno, F., Sawada, R., Shimizu, T., Duan, C., Takahashi, S.I. (2018) Catch-up growth in zebrafish embryo requires neural crest cells sustained by Irs1-signaling. Endocrinology. 159(4):1547-1560
Xin, Y., Duan, C. (2018) Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 1742:205-211
Kamei, H., Duan, C. (2018) Hypoxic Treatment of Zebrafish Embryos and Larvae. Methods in molecular biology (Clifton, N.J.). 1742:195-203
Liu, C., Dai, W., Bai, Y., Chi, C., Xin, Y., He, G., Mai, K., Duan, C. (2017) Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action. Scientific Reports. 7:1994
Zhong, Y., Duan, C. (2017) Lamprey IGF-Binding Protein-3 Has IGF-Dependent and -Independent Actions. Frontiers in endocrinology. 7:174
Zhang, P., Bai, Y., Lu, L., Li, Y., Duan, C. (2016) An oxygen-insensitive Hif-3α isoform inhibits Wnt signaling by destabilizing the nuclear β-catenin complex. eLIFE. 5
Fukushima, T., Yoshihara, H., Furuta, H., Kamei, H., Hakuno, F., Luan, J., Duan, C., Saeki, Y., Tanaka, K., Iemura, S.I., Natsume, T., Chida, K., Nakatsu, Y., Kamata, H., Asano, T., Takahashi, S.I. (2015) Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity. Nature communications. 6:6780
Rong, X., Chen, C., Zhou, P., Zhou, Y., Li, Y., Lu, L., Liu, Y., Zhou, J., Duan, C. (2014) R-Spondin 3 Regulates Dorsoventral and Anteroposterior Patterning by Antagonizing Wnt/β-Catenin Signaling in Zebrafish Embryos. PLoS One. 9(6):e99514
Dai, W., Bai, Y., Hebda, L., Zhong, X., Liu, J., Kao, J., and Duan, C. (2014) Calcium deficiency-induced and TRP channel-regulated IGF1R-PI3K-Akt signaling regulates abnormal epithelial cell proliferation. Cell death and differentiation. 21(4):568-81
Zhang, P., Yao, Q., Lu, L., Li, Y., Chen, P.J., Duan, C. (2014) Hypoxia-inducible factor 3 is an oxygen-dependent transcription activator and regulates a distinct transcriptional response to hypoxia. Cell Reports. 6:1110-21
Liu, C., Luan, J., Bai, Y., Li, Y., Lu, L., Liu, Y., Hakuno, F., Takahashi, S., Duan, C., and Zhou, J. (2014) Aspp2 negatively regulates body growth but not developmental timing by modulating IRS signaling in zebrafish embryos. General and comparative endocrinology. 197:82-91
Zhou, J., Xiang, J., Zhang, S., and Duan, C. (2013) Structural and functional analysis of the amphioxus IGFBP gene uncovers ancient origin of IGF-independent functions. Endocrinology. 154(10):3753-63
Huang, Y., Harrison, M.R., Osorio, A., Kim, J., Baugh, A., Duan, C., Sucov, H.M., and Lien, C.L. (2013) Igf Signaling is Required for Cardiomyocyte Proliferation during Zebrafish Heart Development and Regeneration. PLoS One. 8(6):e67266
Kjaer-Sorensen, K., Engholm, D.H., Kamei, H., Morch, M.G., Kristensen, A.O., Zhou, J., Conover, C.A., Duan, C., and Oxvig, C. (2013) Pregnancy-associated plasma protein-A (PAPP-A) modulates early developmental rate in zebrafish independent of its proteolytic activity. The Journal of biological chemistry. 288(14):9982-92
Rytkönen, K.T., Akbarzadeh, A., Miandare, H.K., Kamei, H., Duan, C., Leder, E.H., Williams, T.A., and Nikinmaa, M. (2013) SUBFUNCTIONALIZATION OF CYPRINID HYPOXIA-INDUCIBLE FACTORS FOR ROLES IN DEVELOPMENT AND OXYGEN SENSING. Evolution; international journal of organic evolution. 67(3):873-882
Jiao, S., Ren, H., Li, Y., Zhou, J., Duan, C., Lu, L. (2013) Differential regulation of IGF-I and IGF-II gene expression in skeletal muscle cells.. Molecular and cellular biochemistry. 373(1):107-113
Zhang, P., Lu, L., Yao, Q., Li, Y., Zhou, J., Liu, Y., and Duan, C. (2012) Molecular, functional, and gene expression analysis of zebrafish hypoxia-inducible factor-3α. American journal of physiology. Regulatory, integrative and comparative physiology. 303(11):R1165-R1174
Onuma, T.A., and Duan, C. (2012) Duplicated Kiss1 receptor genes in zebrafish: distinct gene expression patterns, different ligand selectivity, and a novel nuclear isoform with transactivating activity. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(7):2941-2950
Zhang, C., Lu, L., Li, Y., Wang, X., Zhou, J., Liu, Y., Fu, P., Gallicchio, M.A., Bach, L.A., and Duan, C. (2012) IGF binding protein-6 expression in vascular endothelial cells is induced by hypoxia and plays a negative role in tumor angiogenesis. International Journal of Cancer. 30(9):2003-2012
Feng, Q., Zou, X., Lu, L., Li, Y., Liu, Y., Zhou, J., and Duan, C. (2012) The Stress-Response Gene redd1 Regulates Dorsoventral Patterning by Antagonizing Wnt/β-catenin Activity in Zebrafish. PLoS One. 7(12):e52674
Onuma, T.A., Ding, Y., Abraham, E., Zohar, Y., Ando, H., and Duan, C. (2011) Regulation of Temporal and Spatial Organization of Newborn GnRH Neurons by IGF Signaling in Zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(33):11814-11824
Zhong, Y., Lu, L., Zhou, J., Li, Y., Liu, Y., Clemmons, D.R., and Duan, C. (2011) IGF binding protein 3 exerts its ligand-independent action by antagonizing BMP in zebrafish embryos. Journal of Cell Science. 124(11):1925-1935
Daza, D.O., Sundström, G., Bergqvist, C.A., Duan, C., and Larhammar, D. (2011) Evolution of the insulin-like growth factor binding protein (IGFBP) family. Endocrinology. 152(6):2278-2289
Jiao, S., Dai, W., Lu, L., Liu, Y., Zhou, J., Li, Y., Korzh, V., and Duan, C. (2011) The Conserved Clusterin Gene Is Expressed in the Developing Choroid Plexus Under the Regulation of Notch But Not IGF Signaling in Zebrafish. Endocrinology. 152(5):1860-1871
Kamei, H., Ding, Y., Kajimura, S., Wells, M., Chiang, P., and Duan, C. (2011) Role of IGF signaling in catch-up growth and accelerated temporal development in zebrafish embryos in response to oxygen availability. Development (Cambridge, England). 138(4):777-786
Dai, W., Kamei, H., Zhao, Y., Ding, J., Du, Z., and Duan, C. (2010) Duplicated zebrafish insulin-like growth factor binding protein-5 genes with split functional domains: evidence for evolutionarily conserved IGF binding, nuclear localization, and transactivation activity. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 24(6):2020-2029
Zou, S., Kamei, H., Modi, Z., and Duan, C. (2009) Zebrafish IGF genes: gene duplication, conservation and divergence, and novel roles in midline and notochord development. PLoS One. 4(9):e7026
Bansal, T., Lenhart, J., Kim, T., Duan, C., and Maharbiz, M.M. (2009) Patterned delivery and expression of gene constructs into zebrafish embryos using microfabricated interfaces. Biomedical Microdevices. 11(3):633-641
Wang, X., Lu, L., Li, Y., Li, M., Chen, C., Feng, Q., Zhang, C., and Duan, C. (2009) Molecular and functional characterization of two distinct IGF binding protein-6 genes in zebrafish. American journal of physiology. Regulatory, integrative and comparative physiology. 296(5):R1348-R1357
Li, M., Li, Y., Lu, L., Wang, X., Gong, Q., and Duan, C. (2009) Structural, gene expression, and functional analysis of the Fugu (Takifugu rubripes) insulin-like growth factor binding protein-4 gene. American journal of physiology. Regulatory, integrative and comparative physiology. 296(3):R588-R566
Toyoshima, Y., Monson, C., Duan, C., Wu, Y., Gao, C., Yakar, S., Sadler, K.C., and Leroith, D. (2008) The role of insulin receptor signaling in zebrafish embryogenesis. Endocrinology. 149(12):5996-6005
Kamei, H., Lu, L., Jiao, S., Li, Y., Gyrup, C., Laursen, L.S., Oxvig, C., Zhou, J., and Duan, C. (2008) Duplication and Diversification of the Hypoxia-Inducible IGFBP-1 Gene in Zebrafish. PLoS One. 3(8):e3091
Zhou, J., Li, W., Kamei, H., and Duan, C. (2008) Duplication of the IGFBP-2 gene in teleost fish: protein structure and functionality conservation and gene expression divergence. PLoS One. 3(12):e3926
Schlueter, P.J., Peng, G., Westerfield, M., and Duan, C. (2007) Insulin-like growth factor signaling regulates zebrafish embryonic growth and development by promoting cell survival and cell cycle progression. Cell death and differentiation. 14(6):1095-1105
Schlueter, P.J., Sang, X., Duan, C., and Wood, A.W. (2007) Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival. Developmental Biology. 305(1):377-387
Schlueter, P.J., Royer, T., Farah, M.H., Laser, B., Chan, S.J., Steiner, D.F., and Duan, C. (2006) Gene duplication and functional divergence of the zebrafish insulin-like growth factor 1 receptors. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 20(8):1230-1232
Kajimura, S., Aida, K., and Duan, C. (2006) Understanding hypoxia-induced gene expression in early development: in vitro and in vivo analysis of hypoxia-inducible factor 1-regulated zebra fish insulin-like growth factor binding protein 1 gene expression. Molecular and cellular biology. 26 (3):1142-1155
Wood, A.W., Schlueter, P.J., and Duan, C. (2005) Targeted knockdown of insulin-like growth factor binding protein-2 (IGFBP-2) disrupts cardiovascular development in zebrafish embryos. Molecular endocrinology (Baltimore, Md.). 19(4):1024-1034
Li, Y., Xiang, J., and Duan, C. (2005) Insulin-like Growth Factor-binding Protein-3 Plays an Important Role in Regulating Pharyngeal Skeleton and Inner Ear Formation and Differentiation. The Journal of biological chemistry. 280(5):3613-3620
Kajimura, S., Aida, K., and Duan, C. (2005) Insulin-like growth factor-binding protein-1 (IGFBP-1) mediates hypoxia-induced embryonic growth and developmental retardation. Proceedings of the National Academy of Sciences of the United States of America. 102(4):1240-1245
Wood, A.W., Duan, C., and Bern, H.A. (2005) Insulin-Like Growth Factor Signaling in Fish. International review of cytology. 243:215-285
Duan, C., Ding, J., Schlueter, P.J., Li, Y., Zhang J., and Royer, T. (2003) A zebrafish view of the insulin-like growth factor signaling pathway. Dong wu xue bao. [Acta zoologica Sinica]. 49:421-431
Maures, T.J. and Duan, C. (2002) Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1. Endocrinology. 143(7):2722-2731
Maures, T., Chan, S.-J., Xu, B., Sun, H., Ding, J., and Duan, C. (2002) Structural, biochemical, and expression analysis of two distinct insulin-like growth factor I receptors and their ligands in zebrafish. Endocrinology. 143(5):1858-1871
Duan, C. (2002) Regulation of insulin-like growth factor (IGF) actions by IGF binding protein in zebrafish. Fisheries science : FS. 68:765-768
Pozios, K.C., Ding, J., Degger, B., Upton, Z., and Duan, C. (2001) IGFs stimulate zebrafish cell proliferation by activating MAP kinase and PI3-kinase-signaling pathways. American journal of physiology. Regulatory, integrative and comparative physiology. 280(4):R1230-R1239
Maures, T. and Duan, C. (2000) Expression of insulin-like growth factor binding protein-1 in zebrafish. American zoologist. 40(6):1120
Duan, C.M., Ding, J., Li, Q., Tsai, W., and Pozios, K. (1999) Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 96(26):15274-15279
2004. Yin, P., Xu, Q., and Duan, C. Paradoxical actions of endogenous and exogenous insulin-like growth factor binding protein (IGFBP)-5 revealed by RNA interference analysis. J. Biol. Chem., in press. (published online on May 19, 2004).
2004. Xu, Q., Li, S., Zhao, Y., Maures, T.J., Yin, P., and Duan, C. (2004). Evidence that IGF binding protein-5 functions as a ligand-independent transcriptional regulator in vascular smooth muscle cells. Cir. Res. 94: e46-54.
2004. Xu, Q., Yan, B., Li, S., and Duan, C. (2004). Fibronectin binds insulin-like growth factor binding protein (IGFBP)-5 and abolishes its ligand-dependent action on cell migration. J. Biol. Chem. 279: 4269-4277 (published online as 10.1074/jbc.M311586200 on Nov. 24. 2003).
2003. Hsieh. T. Gordon, R., Clemmons, D.R., Busby, W.H. Jr., and Duan, C. Regulation of the mitogenic and chemotactic responses of vascular smooth muscle cells to insulin-like growth factor (IGF)-I by local IGF binding proteins. J. Biol. Chem. 278: 42886-42892.
2003. Duan, C. The chemotactic and mitogenic responses of vascular smooth muscle cells to insulin-like growth factor-I require the activation of ERK1/2. Mol. Cell. Endocrinol. 206: 75-83.
2002. Duan, C. Specifying the cellular responses to insulin-like growth factor (IGF) signals: Role of IGF binding proteins. J. Endocrinol. 175: 41-54.
2002. Funkenstein, B.I., Tsai, W., Maures, T., and Duan, C. Ontogeny, tissue distribution, and hormonal regulation of insulin-like growth factor binding protein-2 (IGFBP-2) in a marine fish, Sparus aurata. Gen. Comp. Endocrinol. 128: 112-122.
2001. Bauchat, J.R., Busby, W.Jr., Garmany, A., Moore, J., Swanson, P., Lin, M., and Duan, C. Biochemical and functional analysis of a conserved insulin-like growth factor binding protein (IGFBP) isolated from rainbow trout hepatoma cells. J. Endocrinol., 170: 619-628.
2000. Duan, C., Bauchat, J.R. and Hsieh, T. Phosphatidylinositol 3-kinase is required for IGF-I-induced vascular smooth muscle cell proliferation and migration. Cir. Res. 86: 15-23.
1999. Yano, K., Bauchat, J.R., Limatta, M., Clemmons, D.R., and Duan, C. Down-regulation of protein kinase C inhibits insulin-like growth factor-I-induced vascular smooth muscle cell proliferation, migration and gene expression. Endocrinology 140:4622-4632.
1999. Duan, C., Limatta, M.B., and Bottum, O.L. Insulin-like growth factor (IGF)-I regulates IGF binding protein-5 expression through the Phosphatidylinositol-3-Kinase, PKB/Akt and p70 S6 Kinase Signaling Pathway pathway. J. Biol. Chem., 274:37147-37153.
1998. Duan, C. Nutritional and developmental regulation of insulin-like growth factors in fish. J. Nutr., 128: 306S-314S.
1998. Duan, C., and Clemmons, D.R. Differential expression and opposing biological effects of insulin-like growth factor binding protein-4 and -5 in vascular smooth muscle cells. J. Biol. Chem. 273:16836-16842.
1998. Zheng, B., Duan, C., and Clemmons, D.R. The effect of extracellular matrix proteins on porcine smooth muscle cell insulin-like growth factor binding protein-5 synthesis and responsiveness to IGF-I. J. Biol. Chem. 273: 8994-9000.
1997. Duan, C. The insulin-like growth factor system and its biological actions in fish. Am. Zool., 37: 489-501
1996. Duan, C., Hawes, S.B., Prevette, T., and Clemmons, D.R. Insulin-like growth factor (IGF) -I regulates IGF binding protein-5 synthesis through transcriptional activation of the gene in aortic smooth muscle cells. J. Biol. Chem. 271: 4280-4288.
1995. Duan,C., Plisetskaya, E.M. and Dickhoff, W.W. Expression of Insulin-like growth factor-I in normally and abnormally developing (stunting) coho salmon: Relationship with growth and plasma concentrations of growth hormone and insulin. Endocrinology 136: 446-452.
1995. Duan, C. and Clemmons D.R. Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J. Biol. Chem. 270: 24844-24851.