PUBLICATION
Circadian gene period1b regulates proinflammatory cytokine expression through NF-?B signalling in zebrafish
- Authors
- Ren, D.L., Wang, X.B., Hu, B.
- ID
- ZDB-PUB-180701-3
- Date
- 2018
- Source
- Fish & shellfish immunology 80: 528-533 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- Cytokines, ERK, NF-?B, Zebrafish, per1b
- MeSH Terms
-
- Animals
- Circadian Clocks/immunology
- Benzamides/pharmacology
- Inflammation/immunology
- Larva
- Period Circadian Proteins/genetics*
- Period Circadian Proteins/immunology*
- Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
- Extracellular Signal-Regulated MAP Kinases/immunology
- Diphenylamine/analogs & derivatives
- Diphenylamine/pharmacology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/immunology*
- Tail/injuries
- Cytokines/immunology*
- Embryo, Nonmammalian
- Mutation
- Signal Transduction
- NF-kappa B/immunology*
- Zebrafish/genetics*
- Zebrafish/immunology*
- PubMed
- 29958979 Full text @ Fish Shellfish Immunol.
Citation
Ren, D.L., Wang, X.B., Hu, B. (2018) Circadian gene period1b regulates proinflammatory cytokine expression through NF-?B signalling in zebrafish. Fish & shellfish immunology. 80:528-533.
Abstract
The circadian clock plays a critical role in regulating the immune system. Our previous publication revealed that a mutation in the circadian gene period1b (per1b) in zebrafish significantly decreased proinflammatory gene expression, particularly under constant darkness (DD) conditions; however, the underlying mechanisms remain unclear. In this study, using per1b-null mutant zebrafish and a larval tail fin injury model, we observed that the loss of per1b resulted in the downregulation expression of proinflammatory cytokines, such as IL-6 and TNF-?, at protein level. Furthermore, the loss of per1b downregulated ERK phosphorylation and inhibited p65 phosphorylation, leading to reduced NF-?B activation, which could downregulate the expression of proinflammatory cytokines, such as IL-6 and TNF-?, in zebrafish. These results provided insight into the communication between the circadian clock and immune functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping