PUBLICATION

Identification of Ca²⁺ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres.

Authors

Tse, M.K., Hung, T.S., Chan, C.M., Wong, T., Dorothea, M., Leclerc, C., Moreau, M., Miller, A.L., Webb, S.E.

ID

ZDB-PUB-180624-5

Date

2018

Source

Science China. Life sciences 61(11): 1352-1368 (Journal)

Registered Authors

Miller, Andrew L., Webb, Sarah E.

Keywords

Ca2+ signaling, IP3 receptors, STIM and Orai, differentiation, neural stem/progenitor cells, neurospheres, ryanodine receptors, zebrafish

MeSH Terms

Animals
Calcium Signaling/physiology*
Cell Differentiation/physiology*
Cells, Cultured
Gene Expression Regulation, Developmental
Neural Stem Cells/cytology*
Neural Stem Cells/physiology*
Neurodegenerative Diseases/physiopathology
Neuroglia/cytology*
Neurons/cytology*
Signal Transduction
Zebrafish

PubMed

29931586 Full text @ Sci. China Ser. C-Life Sci.

Abstract

The development of the CNS in vertebrate embryos involves the generation of different sub-types of neurons and glia in a complex but highly-ordered spatio-temporal manner. Zebrafish are commonly used for exploring the development, plasticity and regeneration of the CNS, and the recent development of reliable protocols for isolating and culturing neural stem/progenitor cells (NSCs/NPCs) from the brain of adult fish now enables the exploration of mechanisms underlying the induction/specification/differentiation of these cells. Here, we refined a protocol to generate proliferating and differentiating neurospheres from the entire brain of adult zebrafish. We demonstrated via RT-qPCR that some isoforms of ip3r, ryr and stim are upregulated/downregulated significantly in differentiating neurospheres, and via immunolabelling that 1,4,5-inositol trisphosphate receptor (IP3R) type-1 and ryanodine receptor (RyR) type-2 are differentially expressed in cells with neuron- or radial glial-like properties. Furthermore, ATP but not caffeine (IP3R and RyR agonists, respectively), induced the generation of Ca²⁺ transients in cells exhibiting neuron- or glial-like morphology. These results indicate the differential expression of components of the Ca²⁺-signaling toolkit in proliferating and differentiating cells. Thus, given the complexity of the intact vertebrate brain, neurospheres might be a useful system for exploring neurodegenerative disease diagnosis protocols and drug development using Ca²⁺ signaling as a read-out.

Genes / Markers

Figures

Expression

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Tse et al., 2018 ZDB-PUB-180624-5 PMID:29931586

Identification of Ca2+ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres.

Tse et al., 2018

ZDB-PUB-180624-5
PMID:29931586

Identification of Ca²⁺ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres.