PUBLICATION
Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate osteogenic activities in vitro and bone growth in zebrafish larvae
- Authors
- Carson, M.A., Nelson, J., Cancela, M.L., Laizé, V., Gavaia, P.J., Rae, M., Heesch, S., Verzin, E., Maggs, C., Gilmore, B.F., Clarke, S.A.
- ID
- ZDB-PUB-180518-5
- Date
- 2018
- Source
- Scientific Reports 8: 7725 (Journal)
- Registered Authors
- Cancela, Leonor
- Keywords
- none
- MeSH Terms
-
- Zebrafish/growth & development*
- Bone Development/drug effects*
- Cells, Cultured
- In Vitro Techniques
- Osteoporosis/drug therapy*
- Osteoporosis/pathology
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Osteogenesis/drug effects*
- Cell Proliferation
- Cell Differentiation
- Humans
- Larva/drug effects
- Larva/growth & development*
- Plocamium/chemistry
- Rhodophyta/chemistry*
- Mesenchymal Stem Cells/cytology
- Mesenchymal Stem Cells/drug effects
- Animals
- PubMed
- 29769706 Full text @ Sci. Rep.
Citation
Carson, M.A., Nelson, J., Cancela, M.L., Laizé, V., Gavaia, P.J., Rae, M., Heesch, S., Verzin, E., Maggs, C., Gilmore, B.F., Clarke, S.A. (2018) Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate osteogenic activities in vitro and bone growth in zebrafish larvae. Scientific Reports. 8:7725.
Abstract
Through the current trend for bioprospecting, marine organisms - particularly algae - are becoming increasingly known for their osteogenic potential. Such organisms may provide novel treatment options for osteoporosis and other musculoskeletal conditions, helping to address their large healthcare burden and the limitations of current therapies. In this study, extracts from two red algae - Plocamium lyngbyanum and Ceramium secundatum - were tested in vitro and in vivo for their osteogenic potential. In vitro, the growth of human bone marrow stromal cells (hBMSCs) was significantly greater in the presence of the extracts, particularly with P. lyngbyanum treatment. Osteogenic differentiation was promoted more by C. secundatum (70?µg/ml), though P. lyngbyanum had greater in vitro mineralisation potential. Both species caused a marked and dose-dependent increase in the opercular bone area of zebrafish larvae. Our findings therefore indicate the presence of bioactive components in P. lyngbyanum and C. secundatum extracts, which can promote both in vitro and in vivo osteogenic activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping