PUBLICATION
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)
- Authors
- Sander, J.D., Dahlborg, E.J., Goodwin, M.J., Cade, L., Zhang, F., Cifuentes, D., Curtin, S.J., Blackburn, J.S., Thibodeau-Beganny, S., Qi, Y., Pierick, C.J., Hoffman, E., Maeder, M.L., Khayter, C., Reyon, D., Dobbs, D., Langenau, D.M., Stupar, R.M., Giraldez, A.J., Voytas, D.F., Peterson, R.T., Yeh, J.R., Joung, J.K.
- ID
- ZDB-PUB-180321-32
- Date
- 2011
- Source
- Nature Methods 8: 67-9 (Journal)
- Registered Authors
- Cifuentes, Daniel, Giraldez, Antonio, Hoffman, Ellen, Langenau, David, Peterson, Randall, Yeh, Jing-Ruey (Joanna)
- Keywords
- none
- MeSH Terms
-
- Animals
- Arabidopsis/genetics
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Endonucleases/genetics*
- Endonucleases/metabolism*
- Genome
- Glycine max/genetics
- Protein Engineering*
- Zebrafish/genetics
- Zinc Fingers/genetics
- Zinc Fingers/physiology*
- PubMed
- 21151135 Full text @ Nat. Methods
Citation
Sander, J.D., Dahlborg, E.J., Goodwin, M.J., Cade, L., Zhang, F., Cifuentes, D., Curtin, S.J., Blackburn, J.S., Thibodeau-Beganny, S., Qi, Y., Pierick, C.J., Hoffman, E., Maeder, M.L., Khayter, C., Reyon, D., Dobbs, D., Langenau, D.M., Stupar, R.M., Giraldez, A.J., Voytas, D.F., Peterson, R.T., Yeh, J.R., Joung, J.K. (2011) Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nature Methods. 8:67-9.
Abstract
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA-generated ZFNs, we rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping