PUBLICATION
Integrative whole-brain neuroscience in larval zebrafish
- Authors
- Vanwalleghem, G.C., Ahrens, M.B., Scott, E.K.
- ID
- ZDB-PUB-180228-17
- Date
- 2018
- Source
- Current opinion in neurobiology 50: 136-145 (Review)
- Registered Authors
- Ahrens, Misha, Scott, Ethan
- Keywords
- none
- MeSH Terms
-
- Calcium/metabolism
- Neuroimaging
- Microscopy, Fluorescence
- Zebrafish
- Brain*/diagnostic imaging
- Brain*/growth & development
- Brain*/metabolism
- Neurosciences/methods*
- Larva*
- Animals, Genetically Modified
- Animals
- PubMed
- 29486425 Full text @ Curr. Opin. Neurobiol.
Citation
Vanwalleghem, G.C., Ahrens, M.B., Scott, E.K. (2018) Integrative whole-brain neuroscience in larval zebrafish. Current opinion in neurobiology. 50:136-145.
Abstract
Due to their small size and transparency, zebrafish larvae are amenable to a range of fluorescence microscopy techniques. With the development of sensitive genetically encoded calcium indicators, this has extended to the whole-brain imaging of neural activity with cellular resolution. This technique has been used to study brain-wide population dynamics accompanying sensory processing and sensorimotor transformations, and has spurred the development of innovative closed-loop behavioral paradigms in which stimulus-response relationships can be studied. More recently, microscopes have been developed that allow whole-brain calcium imaging in freely swimming and behaving larvae. In this review, we highlight the technologies underlying whole-brain functional imaging in zebrafish, provide examples of the sensory and motor processes that have been studied with this technique, and discuss the need to merge data from whole-brain functional imaging studies with neurochemical and anatomical information to develop holistic models of functional neural circuits.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping