|ZFIN ID: ZDB-PUB-180228-17|
Integrative whole-brain neuroscience in larval zebrafish
Vanwalleghem, G.C., Ahrens, M.B., Scott, E.K.
|Source:||Current opinion in neurobiology 50: 136-145 (Review)|
|Registered Authors:||Ahrens, Misha, Scott, Ethan|
|PubMed:||29486425 Full text @ Curr. Opin. Neurobiol.|
Vanwalleghem, G.C., Ahrens, M.B., Scott, E.K. (2018) Integrative whole-brain neuroscience in larval zebrafish. Current opinion in neurobiology. 50:136-145.
ABSTRACTDue to their small size and transparency, zebrafish larvae are amenable to a range of fluorescence microscopy techniques. With the development of sensitive genetically encoded calcium indicators, this has extended to the whole-brain imaging of neural activity with cellular resolution. This technique has been used to study brain-wide population dynamics accompanying sensory processing and sensorimotor transformations, and has spurred the development of innovative closed-loop behavioral paradigms in which stimulus-response relationships can be studied. More recently, microscopes have been developed that allow whole-brain calcium imaging in freely swimming and behaving larvae. In this review, we highlight the technologies underlying whole-brain functional imaging in zebrafish, provide examples of the sensory and motor processes that have been studied with this technique, and discuss the need to merge data from whole-brain functional imaging studies with neurochemical and anatomical information to develop holistic models of functional neural circuits.
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