PUBLICATION

Activating the regenerative potential of Müller glia cells in a regeneration-deficient retina

Authors
Lust, K., Wittbrodt, J.
ID
ZDB-PUB-180130-11
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Wittbrodt, Jochen
Keywords
developmental biology, stem cells, zebrafish
MeSH Terms
  • Animals
  • Cell Proliferation
  • Cellular Reprogramming
  • Ependymoglial Cells/physiology*
  • Oryzias/physiology*
  • Regeneration*
  • Retina/injuries
  • Retina/physiology*
  • Zebrafish/physiology*
PubMed
29376827 Full text @ Elife
Abstract
Regeneration responses in animals are widespread across phyla. To identify molecular players that confer regenerative capacities to non-regenerative species is of key relevance for basic research and translational approaches. Here, we report a differential response in retinal regeneration between medaka (Oryzias latipes) and zebrafish (Danio rerio). In contrast to zebrafish, medaka Müller glia (olMG) cells behave like progenitors and exhibit a restricted capacity to regenerate the retina. After injury, olMG cells proliferate but fail to self-renew and ultimately only restore photoreceptors. In our injury paradigm, we observed that in contrast to zebrafish, proliferating olMG cells do not maintain sox2 expression. Sustained sox2 expression in olMG cells confers regenerative responses similar to those of zebrafish MG (drMG) cells. We show that a single, cell-autonomous factor reprograms olMG cells and establishes a regeneration-like mode. Our results position medaka as an attractive model to delineate key regeneration factors with translational potential.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping