PUBLICATION

Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum

Authors
Theisen, U., Hennig, C., Ring, T., Schnabel, R., Köster, R.W.
ID
ZDB-PUB-180105-3
Date
2018
Source
PLoS Biology   16: e2002226 (Journal)
Registered Authors
Köster, Reinhard W., Theisen, Ulrike
Keywords
none
MeSH Terms
  • Glutamic Acid/metabolism
  • Glycine/metabolism
  • Brain
  • Embryonic Development/physiology
  • Cerebellum/physiology
  • Zebrafish/embryology
  • Neurons/physiology*
  • Calcium/metabolism
  • Neurogenesis/physiology*
  • Cell Differentiation/physiology
  • Optogenetics/methods
  • Cell Movement/physiology*
  • Animals
  • Acetylcholine/metabolism
  • Neurotransmitter Agents/metabolism
  • Calcium Signaling/physiology
  • Brain Mapping
(all 17)
PubMed
29300740 Full text @ PLoS Biol.
Abstract
Neuronal migration during embryonic development contributes to functional brain circuitry. Many neurons migrate in morphologically distinct stages that coincide with differentiation, requiring tight spatial regulation. It had been proposed that neurotransmitter-mediated activity could exert this control. Here, we demonstrate that intracellular calcium transients occur in cerebellar neurons of zebrafish embryos during migration. We show that depolarization increases and hyperpolarization reduces the speed of tegmental hindbrain neurons using optogenetic tools and advanced track analysis optimized for in vivo migration. Finally, we introduce a compound screening assay to identify acetylcholine (ACh), glutamate, and glycine as regulators of migration, which act regionally along the neurons' route. We summarize our findings in a model describing how different neurotransmitters spatially interact to control neuronal migration. The high evolutionary conservation of the cerebellum and hindbrain makes it likely that polarization state-driven motility constitutes an important principle in building a functional brain.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
GAL4TA4EFGGAL4TA4
GCaMPEFGGCaMP
GFPEFGGFP
1 - 3 of 3
Show
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Theisen, U., Hennig, C., Ring, T., Schnabel, R., Köster, R.W. (2018) Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum. PLoS Biology. 16:e2002226.