PUBLICATION

Leucine repeat adaptor protein 1 interacts with Dishevelled to regulate gastrulation cell movements in zebrafish

Authors
Cheng, X.N., Shao, M., Li, J.T., Wang, Y.F., Qi, J., Xu, Z.G., Shi, D.L.
ID
ZDB-PUB-171109-4
Date
2017
Source
Nature communications   8: 1353 (Journal)
Registered Authors
Shao, Ming, Shi, De-Li
Keywords
none
MeSH Terms
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism*
  • Animals
  • Cell Movement
  • Cell Polarity
  • Dishevelled Proteins/genetics
  • Dishevelled Proteins/metabolism*
  • Embryo, Nonmammalian
  • Female
  • Gastrula/cytology*
  • Gene Expression Regulation, Developmental
  • Humans
  • MAP Kinase Kinase 4/metabolism
  • Mutation
  • Signal Transduction
  • Wnt Proteins/metabolism
  • Xenopus
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 20)
PubMed
29116181 Full text @ Nat. Commun.
Abstract
Gastrulation is a fundamental morphogenetic event that requires polarised cell behaviours for coordinated asymmetric cell movements. Wnt/PCP signalling plays a critical role in this process. Dishevelled is an important conserved scaffold protein that relays Wnt/PCP signals from membrane receptors to the modulation of cytoskeleton organisation. However, it remains unclear how its activity is regulated for the activation of downstream effectors. Here, we report that Lurap1 is a Dishevelled-interacting protein that regulates Wnt/PCP signalling in convergence and extension movements during vertebrate gastrulation. Its loss-of-function leads to enhanced Dishevelled membrane localisation and increased JNK activity. In maternal-zygotic lurap1 mutant zebrafish embryos, cell polarity and directional movement are disrupted. Time-lapse analyses indicate that Lurap1, Dishevelled, and JNK functionally interact to orchestrate polarised cellular protrusive activity, and Lurap1 is required for coordinated centriole/MTOC positioning in movement cells. These findings demonstrate that Lurap1 functions to regulate cellular polarisation and motile behaviours during gastrulation movements.
Genes / Markers
Marker Marker Type Name
cetn4GENEcentrin 4
chrdGENEchordin
dlx3bGENEdistal-less homeobox 3b
dvl2GENEdishevelled segment polarity protein 2
dvl3aGENEdishevelled segment polarity protein 3a
egr2bGENEearly growth response 2b
eve1GENEeven-skipped-like1
lurap1GENEleucine rich adaptor protein 1
myod1GENEmyogenic differentiation 1
pax2aGENEpaired box 2a
1 - 10 of 14
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Figures
Figure Gallery (15 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m209
    Point Mutation
    sdu20
      Indel
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      dvl2MO3-dvl2MRPHLNO
      dvl3aMO1-dvl3aMRPHLNO
      lurap1TALEN1-lurap1TALEN
      1 - 3 of 3
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      Fish
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-mycmonoclonal
        IgG1Mouse
        1 - 1 of 1
        Show
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available