PUBLICATION
Modelling acrylamide acute neurotoxicity in zebrafish larvae
- Authors
- Prats, E., Gómez-Canela, C., Ben-Lulu, S., Ziv, T., Padrós, F., Tornero, D., Garcia-Reyero, N., Tauler, R., Admon, A., Raldúa, D.
- ID
- ZDB-PUB-171028-20
- Date
- 2017
- Source
- Scientific Reports 7: 13952 (Journal)
- Registered Authors
- Raldúa, Demetrio
- Keywords
- none
- MeSH Terms
-
- Acrylamide/toxicity*
- Animals
- Larva/drug effects*
- Larva/genetics
- Models, Biological*
- Neurotoxins/toxicity*
- Transcription, Genetic/drug effects
- Zebrafish*
- PubMed
- 29066856 Full text @ Sci. Rep.
Citation
Prats, E., Gómez-Canela, C., Ben-Lulu, S., Ziv, T., Padrós, F., Tornero, D., Garcia-Reyero, N., Tauler, R., Admon, A., Raldúa, D. (2017) Modelling acrylamide acute neurotoxicity in zebrafish larvae. Scientific Reports. 7:13952.
Abstract
Acrylamide (ACR), a type-2 alkene, may lead to a synaptopathy characterized by ataxia, skeletal muscles weakness and numbness of the extremities in exposed human and laboratory animals. Currently, only the mildly affected patients undergo complete recovery, and identification of new molecules with therapeutic bioactivity against ACR acute neurotoxicity is urgently needed. Here, we have generated a zebrafish model for ACR neurotoxicity by exposing 5 days post-fertilization zebrafish larvae to 1 mM ACR for 3 days. Our results show that zebrafish mimics most of the pathophysiological processes described in humans and mammalian models. Motor function was altered, and specific effects were found on the presynaptic nerve terminals at the neuromuscular junction level, but not on the axonal tracts or myelin sheath integrity. Transcriptional markers of proteins involved in synaptic vesicle cycle were selectively altered, and the proteomic analysis showed that ACR-adducts were formed on cysteine residues of some synaptic proteins. Finally, analysis of neurotransmitters profile showed a significant effect on cholinergic and dopaminergic systems. These data support the suitability of the developed zebrafish model for screening of molecules with therapeutic value against this toxic neuropathy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping