PUBLICATION
A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka
- Authors
- Yu, T., Graf, M., Renn, J., Schartl, M., Larionova, D., Huysseune, A., Witten, P.E., Winkler, C.
- ID
- ZDB-PUB-170928-21
- Date
- 2017
- Source
- Development (Cambridge, England) 144: 265-271 (Journal)
- Registered Authors
- Graf, Martin, Huysseune, Ann, Renn, Joerg, Schartl, Manfred, Winkler, Christoph, Witten, P. Eckhard
- Keywords
- Bone modelling, Medaka, Osteoblasts, Osteogenesis, Skeleton
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Calcification, Physiologic/genetics
- Gene Expression Regulation, Developmental
- Gene Knockout Techniques
- Notochord/embryology
- Oryzias/embryology*
- Oryzias/genetics*
- Osteogenesis/genetics*
- Phylogeny
- Species Specificity
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Vertebrates/embryology
- Vertebrates/genetics
- Zebrafish Proteins/physiology
- PubMed
- 27993982 Full text @ Development
Citation
Yu, T., Graf, M., Renn, J., Schartl, M., Larionova, D., Huysseune, A., Witten, P.E., Winkler, C. (2017) A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka. Development (Cambridge, England). 144:265-271.
Abstract
osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping